Flow cytometric analysis of CD4 expression on Human PBMCs (peripheral blood mononuclear cells). Human PBMCs were stained with Brilliant Violet 421™ Mouse Anti-Human CD3 Antibody and either Biotin Mouse IgG1, κ Isotype Control (left panel) or SDT Biotin Mouse Anti-Human CD4 Antibody (right panel) at 5 μl/test followed by Sav-iFluor 488. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Biotin Mouse Anti-Human CD4 Antibody (RPA-T4)
Biotin Mouse Anti-Human CD4 Antibody (RPA-T4)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD4 |
| Synonyms | T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3 |
| Location | Cell membrane |
| Accession | P01730 |
| Clone Number | RPA-T4 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Isotype Control | S0B5431 |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | Human PBMC |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Biotin |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Hu |
Background
CD4 is a glycoprotein found on the surface of certain immune cells, such as helper T cells, monocytes, macrophages, and dendritic cells. It is a monomeric type I transmembrane molecule composed of four immunoglobulin-like domains (D1 to D4) and encoded by a single gene. As a co-receptor for the T-cell receptor (TCR), CD4 plays a crucial role in the immune response by binding to the MHC class II molecules on antigen-presenting cells, which helps T cells recognize and respond to foreign antigens. The interaction between CD4 and MHC class II molecules also activates downstream signaling pathways, leading to the activation of transcription factors and promoting T cell activation. Additionally, CD4 is significant in HIV infection, as the virus binds to CD4 to enter host cells.
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