Flow cytometric analysis of Human CD235a/b expression on Human Peripheral Blood cells. Human Peripheral Blood cells were stained with PE-Cy7 Mouse Anti-Human CD45 Antibody and either Biotin Mouse IgG2b, κ Isotype Control (Left panel) or Biotin Mouse Anti-Human CD235a/b Antibody (Right panel) at 1.25 μl/test followed by Sav-iFluor 488. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Biotin Mouse Anti-Human CD235a/b Antibody (S-R403)
Biotin Mouse Anti-Human CD235a/b Antibody (S-R403)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD235a |
| Synonyms | Glycophorin-A; MN sialoglycoprotein; PAS-2; Sialoglycoprotein alpha; GYPA; GPA; MNS; Glycophorin-B; PAS-3; SS-active sialoglycoprotein; Sialoglycoprotein delta; GPB; GYPB |
| Location | Cell membrane |
| Accession | P02724、P06028 |
| Clone Number | S-R403 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2b,k |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | Human Peripheral Blood cells |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | Biotin |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1.25μl per million cells in 100μl volume | Hu |
Background
CD235a/b proteins, also known as glycophorins A and B, are type I transmembrane sialoglycoproteins expressed on human erythrocytes, erythroid precursor cells, and certain leukemic cell types. CD235a (glycophorin A) is a single-pass transmembrane glycoprotein with a molecular weight of approximately 10 kD, present in the cell membrane as a homodimer. It serves as the carrier for blood group M and N specificities and plays a role in reducing red blood cell aggregation in circulation. Additionally, CD235a acts as a receptor for certain viruses and bacterial adhesins, such as Sandei virus, parvovirus, and Streptococcus adhesin Hsa.
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