Flow cytometric analysis of Human CD235a expression on human PBMC (human peripheral blood mononuclear cell). Human PBMC were stained with SDT Biotin Mouse Anti-Human CD235a Antibody (Red line histogram) at 1 μg/test followed by Sav-PE, cells without incubation with primary antibody and secondary antibody (Black line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Biotin Mouse Anti-Human CD235a Antibody (S-R473)
Biotin Mouse Anti-Human CD235a Antibody (S-R473)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD235a |
| Synonyms | Glycophorin-A; MN sialoglycoprotein; PAS-2; Sialoglycoprotein alpha; GPA; GYPA |
| Location | Cell membrane |
| Accession | P02724 |
| Clone Number | S-R473 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2a,k |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | Human PBMC |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | Biotin |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 5 μl per million cells in 100μl volume | Hu |
Background
CD235a, also known as Glycophorin A, is the most abundant sialoglycoprotein on the surface of human erythrocytes. It plays a crucial role in reducing red blood cell aggregation and maintains blood fluidity. CD235a serves as a receptor for various pathogens, including the influenza virus, Plasmodium falciparum (the parasite causing malaria), and hepatitis A virus, making it significant in the context of infectious diseases. Its expression and function are essential for the proper physiological function of red blood cells.
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