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Biotin Mouse Anti-Human CD170 (Siglec-5) Antibody (S-4097)

Biotin Mouse Anti-Human CD170 (Siglec-5) Antibody (S-4097)

Catalog Number: S0B8878 Application: FCM Reactivity: Human Conjugation: Biotin Brand: Starter
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Regular price $195 USD
Regular price Sale price $195 USD
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Product Details

Product Specification


Host Mouse
Antigen CD170 (Siglec-5)
Synonyms Sialic acid-binding Ig-like lectin 5; CD33 antigen-like 2; Obesity-binding protein 2 (OB-BP2; OB-binding protein 2); SIGLEC5; CD33L2; OBBP2
Location Membrane
Accession O15389
Clone Number S-4097
Antibody Type Mouse mAb
Isotype IgG1,k
Application FCM
Reactivity Hu
Positive Sample Human Peripheral Blood cells
Purification Protein G
Concentration 0.2 mg/ml
Conjugation Biotin
Physical Appearance Liquid
Storage Buffer

PBS pH7.4, 0.03% Proclin 300

Stability & Storage

12 months from date of receipt / reconstitution, 2 to 8 °C as supplied

Dilution


application dilution species
FCM 5μl per million cells in 100μl volume Hu

Background

CD170 protein, also known as sialic acid-binding immunoglobulin-like lectin-5 (Siglec-5), is a type I transmembrane inhibitory receptor primarily expressed on myeloid cells (such as monocytes, macrophages, and neutrophils) and some B cells. It belongs to the immunoglobulin superfamily and the Siglec family. Its extracellular region contains an immunoglobulin-like domain that specifically recognizes and binds to sialylated glycans (commonly found on host cell surfaces), while its intracellular region contains an immunoreceptor tyrosine-based inhibitory motif (ITIM). Upon binding to sialylated self-ligands, the ITIM domain of CD170 becomes phosphorylated, recruiting and activating SH2 domain-containing protein tyrosine phosphatases (e.g., SHP-1/SHP-2), thereby transmitting potent inhibitory signals that negatively regulate the activation of myeloid cells, the production of inflammatory cytokines, and phagocytic functions. This mechanism plays a critical role in maintaining immune homeostasis and preventing excessive inflammatory responses against self-tissues. In pathological conditions, certain pathogens or tumor cells can evade immune surveillance by engaging CD170 through "molecular mimicry" or "decoy" mechanisms using surface sialylated glycans, thereby promoting persistent infection or tumor progression. Thus, CD170 is not only a key regulator of immune self-tolerance but also a potential target for infection immunotherapy and cancer immunotherapy. Modulating its function—for example, using monoclonal antibodies for blockade or agonism—may offer novel therapeutic strategies for autoimmune diseases, chronic inflammation, and cancer.

Picture

FC

Flow cytometric analysis of human CD170 expression on human peripheral blood cells. Human peripheral blood cells were stained with Allophycocyanin Mouse Anti-Human CD15 Antibody and either Biotin IgG1, k Isotype Control (left panel) or SDT Biotin Mouse Anti-Human CD170 Antibody (right panel) at 5 μl/test followed by Sav-iFluor 488. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.