Flow cytometric analysis of CD16a expression on human PBMC (human peripheral blood mononuclear cells) populations. Human PBMC were stained with Brilliant Violet 421™ mouse anti-human CD3 Antibody and either Biotin Mouse IgG1, κ Isotype Control (left panel) or SDT Biotin Mouse Anti- Human CD16a Antibody (right panel) at 5 μl/test followed by Sav-iFluor 488. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Biotin Mouse Anti-Human CD16a Antibody (S-564-39)
Biotin Mouse Anti-Human CD16a Antibody (S-564-39)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD16a |
| Synonyms | Low affinity immunoglobulin gamma Fc region receptor III-A; IgG Fc receptor III-A; CD16-II; CD16a antigen; Fc-gamma RIII-alpha (Fc-gamma RIII; Fc-gamma RIIIa; FcRIII; FcRIIIa; FcgammaRIIIA); FcR-10; IgG Fc receptor III-2; FCG3; FCGR3; IGFR3; FCGR3A |
| Immunogen | Recombinant Protein |
| Location | Secreted, Cell membrane |
| Accession | P08637 |
| Clone Number | S-564-39 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | Human PBMC |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Biotin |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Hu |
Background
CD16, also known as FcγRIII, is a type of glycoprotein belonging to the immunoglobulin superfamily. It is divided into two subtypes: CD16a (FcγRIIIa) and CD16b (FcγRIIIb). CD16a is a transmembrane protein mainly expressed on natural killer (NK) cells, monocytes, and some T cell subsets, while CD16b is a GPI-anchored protein primarily found on neutrophils. CD16a can mediate antibody-dependent cell cytotoxicity (ADCC) by binding to the Fc region of IgG antibodies, which is crucial for the immune system to eliminate virus-infected cells and cancer cells. Additionally, CD16a is one of the core markers for defining NK cells and can promote their proliferation and secretion of cytotoxic cytokines. In cancer immunotherapy, CD16a is an important target protein, and some monoclonal antibody drugs are designed to enhance its binding ability to boost immune responses.
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