Flow cytometric analysis of human CD158bj expression on human peripheral blood lymphocytes. Human peripheral blood lymphocytes were stained with PE Mouse Anti-Human CD56 Antibody and either Biotin Mouse IgG2a, κ Isotype Control (left panel) or SDT Biotin Mouse Anti-Human CD158bj Antibody (right panel) at 5 μl/test followed by Sav-iFluor 488. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Biotin Mouse Anti-Human CD158b/j Antibody (S-2917)
Biotin Mouse Anti-Human CD158b/j Antibody (S-2917)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD158b/j |
| Synonyms | CD158b1; KIR2DL2; CD158B1; NKAT2a; NKAT2b; CD158b2; KIR2DL3; CD158B2; KIRCL23; NKAT2; CD158j; KIR2DS2; CD158J; NKAT5 |
| Location | Cell membrane |
| Accession | P43627 |
| Clone Number | S-2917 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2a,k |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | human peripheral blood lymphocytes |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | Biotin |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Hu |
Background
CD158b/j, also known as KIR2DL2/L3/S2, is a member of the killer cell immunoglobulin-like receptor (KIR) family and belongs to the immunoglobulin superfamily. It is a type I membrane glycoprotein with two immunoglobulin C2-type domains. CD158b/j is expressed on natural killer (NK) cells and a subset of T cells. These receptors play a crucial role in modulating the immune response by interacting with specific HLA-C antigens on target cells, thereby inhibiting the cytotoxic functions of NK and T cells. The expression of CD158b/j on T cells increases with age, particularly in CD8 T cells, due to epigenetic changes such as DNA demethylation. This age-related expression pattern may contribute to the immune senescence observed in older individuals.
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