Flow cytometric analysis of CD144 expression on HUVEC (human umbilical vein endothelial cell). Cells from HUVEC were stained with either Biotin Mouse IgG1, κ Isotype Control (black line histogram) or SDT Biotin Mouse Anti-Human CD144 Antibody (red line histogram) at 5 μl/test followed by Sav-iFluor 488, cells without incubation with primary antibody and secondary antibody (blue line histogram) was used as unlabeled control. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Biotin Mouse Anti-Human CD144 Antibody (S-3026)
Biotin Mouse Anti-Human CD144 Antibody (S-3026)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD144 |
| Synonyms | Cadherin-5; 7B4 antigen; Vascular endothelial cadherin (VE-cadherin); CDH5 |
| Location | Cell membrane, Cell junction |
| Accession | P33151 |
| Clone Number | S-3026 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | HUVEC |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Biotin |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Hu |
Background
CD144, also termed VE-cadherin or cadherin-5, is a 140 kDa calcium-dependent type-II classical cadherin expressed almost exclusively on vascular and lymphatic endothelial cells, where it forms the core of adherens junctions by mediating homophilic trans-interactions between neighboring cells and anchoring to the actin cytoskeleton via catenins (β-, γ-, and p120-catenin), thereby controlling endothelial barrier integrity, vascular permeability, leukocyte extravasation, and embryonic angiogenesis, while also modulating VEGF receptor signaling, cell polarity, and lumen formation .
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