Flow cytometric analysis of Bcl-6 expression in Jurkat (Human T cell leukemia T lymphocyte, left) / Ramos (Human Burkitt's lymphoma B lymphocyte, right). Jurkat / Ramos were fixed and permeabilized with Foxp3 / Transcription Factor Staining Buffer Set. The cells were then stained with either Biotin Mouse IgG1, k Isotype Control (black line histogram) or SDT Biotin Mouse Anti-Human Bcl-6 Antibody (red line histogram) at 5 μl/test followed by Sav-PE. Total viable cells, as determined by Fixable Viability Dye 545 (S0B88802). Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Biotin Mouse Anti-Human BCL-6 Antibody (S-R634)
Biotin Mouse Anti-Human BCL-6 Antibody (S-R634)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | BCL-6 |
| Synonyms | B-cell lymphoma 6 protein; BCL-6; B-cell lymphoma 5 protein (BCL-5); Protein LAZ-3; Zinc finger and BTB domain-containing protein 27; Zinc finger protein 51; BCL5; LAZ3; ZBTB27; ZNF51; BCL6 |
| Location | Nucleus |
| Accession | P41182 |
| Clone Number | S-R634 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | ICFCM |
| Reactivity | Hu |
| Positive Sample | Ramos |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Biotin |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| ICFCM | 5μl per million cells in 100μl volume | Hu |
Background
BCL-6 protein is a transcriptional repressor that plays a crucial role in the regulation of B cell differentiation and proliferation. It is primarily expressed in germinal center B cells and is essential for the formation and function of germinal centers, which are specialized microenvironments within lymphoid tissues where B cells undergo somatic hypermutation and class switch recombination to generate high-affinity antibodies. BCL-6 achieves its regulatory functions by binding to specific DNA sequences in the promoters of target genes, thereby inhibiting their transcription. Abnormal expression or function of BCL-6 is associated with various B cell lymphomas, making it an important biomarker and therapeutic target in the study and treatment of these malignancies.
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