The Biotin-KRAS[Q61L] activity was detected using HTRF technology. The reaction was performed by incubating the Biotin-KRAS[Q61L] protein and substrate, adding the beads and keeping the mixture at 25℃ for 60 min, then reading ratio 665/620 with BMG.
Product Details
Product Details
Product Specification
| Species | Human |
| Accession | P01116-2 |
| Amino Acid Sequence | M1-K169;N-terminal 8x His tag, C-terminal Avi tag |
| Expression System | E.coli |
| Molecular Weight | 23.5 kDa |
| Purity | > 95% by SDS-PAGE |
| Conjugation | Unconjugated |
| Tag | Avi Tag, His Tag |
| Physical Appearance | Liquid |
| Storage Buffer | 50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5 |
| Stability & Storage | Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles. |
Picture
Picture
Bioactivity
SDS-PAGE
![Biotin-KRAS[Q61L] Protein, Human(Load GDP/GMPPNP)](http://www.antbioinc.com/cdn/shop/files/AntBioImage_9c729f7c-263e-436a-a49a-195cb6b6bd14.png?v=1776073560&width=1445)