Flow cytometric analysis of CD29 expression on C57BL/6 mouse bone marrow. C57BL/6 mouse bone marrow was stained with Alexa Fluor® 647 Rat Anti-Mouse CD45R/B220 Antibody and either Biotin Armenian hamster IgG Isotype Control (left panel) or SDT Biotin Armenian hamster Anti-Mouse/Rat CD29 Antibody (right panel) at 2 μl/test followed by Sav-iFluor 488. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Biotin Armenian hamster Anti-Mouse/Rat CD29 (HMB1-1)
Biotin Armenian hamster Anti-Mouse/Rat CD29 (HMB1-1)
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Product Details
Product Details
Product Specification
| Host | Armenian hamster |
| Antigen | CD29 |
| Synonyms | Integrin beta-1; Fibronectin receptor subunit beta; VLA-4 subunit beta; Itgb1 |
| Location | Endosome, Cell membrane |
| Accession | P09055、 P49134 |
| Clone Number | HMB1-1 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | FCM |
| Reactivity | Ms, Rt |
| Positive Sample | C57BL/6 mouse bone marrow |
| Purification | Protein G |
| Concentration | 0.5 mg/ml |
| Conjugation | Biotin |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1μg per million cells in 100μl volume | Ms, Rt |
Background
CD29, also known as integrin beta-1 (ITGB1), is a crucial cell surface receptor involved in cell adhesion, migration, and signaling. It is a member of the integrin family and is encoded by the ITGB1 gene, located on human chromosome 10p11.2. CD29 forms complexes with various integrin alpha subunits to interact with extracellular matrix (ECM) proteins such as collagen, laminin, and fibronectin. This protein plays a significant role in cellular processes including “inside-out” and “outside-in” signaling pathways, which are essential for cell survival and metastasis. Additionally, CD29 is highly expressed in various cell types and is implicated in multiple diseases, including cancer progression and immune regulation.
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