WB result of BIN1 Recombinant Rabbit mAb
Primary antibody: BIN1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Daudi whole cell lysate 20 µg
Lane 2: U-87 MG whole cell lysate 20 µg
Lane 3: SK-OV-3 whole cell lysate 20 µg
Lane 4: HeLa whole cell lysate 20 µg
Lane 5: A172 whole cell lysate 20 µg
Negative control: Daudi whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 64 kDa
Observed MW: 50-70 kDa
BIN1 Recombinant Rabbit mAb (S-4287-18)
BIN1 Recombinant Rabbit mAb (S-4287-18)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | BIN1 |
| Synonyms | Myc box-dependent-interacting protein 1; Amphiphysin II; Amphiphysin-like protein; Box-dependent myc-interacting protein 1; Bridging integrator 1; AMPHL |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | O00499 |
| Clone Number | S-4287-18 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:2000 | Hu, Ms, Rt |
Background
BIN1 (Bridging Integrator 1) is a multifunctional adaptor protein encoded by the BIN1 gene, belonging to the BAR (Bin/Amphiphysin/Rvs) domain protein family, and plays multiple critical roles in cells including membrane remodeling, vesicle trafficking, and cytoskeletal regulation. Its most classic function involves sensing and inducing membrane curvature through its N-terminal BAR domain, thereby participating in clathrin-mediated endocytosis and the formation of tubular membrane structures, which are essential for synaptic vesicle recycling and T-tubule biogenesis in muscle cells. BIN1 is expressed in various tissues and generates multiple isoforms through alternative splicing, among which the muscle-specific isoforms are indispensable for maintaining the structure and function of skeletal and cardiac muscle, and mutations in this gene are closely associated with autosomal dominant centronuclear myopathy. Furthermore, BIN1 is widely studied as a risk gene for Alzheimer's disease, where its functional alterations may contribute to disease progression by affecting neuronal endocytosis and tau pathology. In addition, it exhibits a dual role in various tumors—acting as a tumor suppressor by inhibiting c-Myc transcriptional activity while potentially promoting tumor invasion under certain contexts—making it a cross-disciplinary hotspot protein that connects neuroscience, muscle diseases, and oncology.
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Western Blot
WB result of BIN1 Recombinant Rabbit mAb
Primary antibody: BIN1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C2C12 whole cell lysate 20 µg
Lane 2: mouse skeletal muscle lysate 20 µg
Lane 3: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 64 kDa
Observed MW: 45-80 kDa
WB result of BIN1 Recombinant Rabbit mAb
Primary antibody: BIN1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat skeletal muscle lysate 20 µg
Lane 3: rat brain lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 64 kDa
Observed MW: 45-80 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-BIN1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human skeletal muscle. Anti-BIN1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver cancer. Anti-BIN1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-BIN1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti-BIN1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-BIN1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse skeletal muscle. Anti-BIN1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat skeletal muscle. Anti-BIN1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
