WB result of Beta III Tubulin Recombinant Rabbit mAb
Primary antibody: Beta III Tubulin Recombinant Rabbit mAb at 1/30000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HEK-293 whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Lane 4: Jurkat whole cell lysate 20 µg
Lane 5: MCF7 whole cell lysate 20 µg
Lane 6: HT-1080 whole cell lysate 20 µg
Lane 7: LNCaP whole cell lysate 20 µg
Lane 8: SH-SY5Y whole cell lysate 20 µg
Lane 9: U-87 MG whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 50 kDa
Observed MW: 52 kDa
Beta III Tubulin Recombinant Rabbit mAb (S-5529)
Beta III Tubulin Recombinant Rabbit mAb (S-5529)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Beta III Tubulin |
| Synonyms | Tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-III; TUBB4; TUBB3 |
| Location | Cytoplasm, Cytoskeleton |
| Accession | Q13509 |
| Clone Number | S-5529 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, HEK-293, HepG2, Jurkat, MCF7, HT-1080, LNCaP, SH-SY5Y, U-87 MG, NIH/3T3, C2C12, Neuro-2a, mouse brain, PC-12, rat brain |
| Purification | Protein A |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:5000-1:50000 | Hu, Ms, Rt |
| IHC-P | 1:2000-1:4000 | Hu, Ms, Rt |
| ICC | 1:1000 | Hu, Ms, Rt |
Background
Beta III Tubulin (βIII-tubulin, encoded by the TUBB3 gene) is a highly specialized β-tubulin isotype within the tubulin protein family. Its most distinctive feature is its highly restricted expression under normal physiological conditions—it is found almost exclusively in the testes and in tissues derived from the neural crest, which has led to its widespread use as a classic marker for neural development and neuronal lineage identification. However, the "identity" of this protein undergoes a dramatic shift in disease states: in various epithelial-derived tumors such as lung cancer, breast cancer, and ovarian cancer, βIII-tubulin is aberrantly overexpressed and is closely associated with increased tumor aggressiveness and poor patient prognosis. At the functional level, it may play specialized roles in normal neurons, potentially by enhancing microtubule dynamic instability or providing protection against reactive oxygen species. On the other hand, its overexpression is also a major mechanism underlying cancer cell resistance to microtubule-targeting drugs such as taxanes, making it a highly promising prognostic biomarker for cancer and a therapeutic target for overcoming drug resistance.
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Western Blot
WB result of Beta III Tubulin Recombinant Rabbit mAb
Primary antibody: Beta III Tubulin Recombinant Rabbit mAb at 1/30000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: C2C12 whole cell lysate 20 µg
Lane 3: Neuro-2a whole cell lysate 20 µg
Lane 4: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 50 kDa
Observed MW: 52 kDa
WB result of Beta III Tubulin Recombinant Rabbit mAb
Primary antibody: Beta III Tubulin Recombinant Rabbit mAb at 1/30000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 50 kDa
Observed MW: 55 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human brain. Anti-Beta III tubulin antibody was used at 1/4000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon. Anti-Beta III tubulin antibody was used at 1/4000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-Beta III tubulin antibody was used at 1/4000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach cancer. Anti-Beta III tubulin antibody was used at 1/4000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse brain. Anti-Beta III tubulin antibody was used at 1/4000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat brain. Anti-Beta III tubulin antibody was used at 1/4000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti- Beta III tubulin antibody was used at 1/1000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
ICC shows positive staining in C2C12 cells. Anti- Beta III tubulin antibody was used at 1/1000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
ICC shows positive staining in C6 cells. Anti- Beta III tubulin antibody was used at 1/1000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
