Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized SH-SY5Y (Human neuroblastoma epithelial cell) cells labelling Beta III tubulin Recombinant Rabbit mAb (Alexa Fluor® 488 Conjugate) at 1/200 (0.5 μg) dilution / (red) compared with a Rabbit monoclonal IgG (black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Beta III tubulin Recombinant Rabbit mAb (Alexa Fluor® 488 Conjugate) (SDT-251-28)
Beta III tubulin Recombinant Rabbit mAb (Alexa Fluor® 488 Conjugate) (SDT-251-28)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Beta III Tubulin |
| Synonyms | Tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-III; TUBB4; TUBB3 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Cytoskeleton |
| Accession | Q13509 |
| Clone Number | SDT-251-28 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | ICC, IF, ICFCM |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | SH-SY5Y |
| Purification | Protein A |
| Concentration | 1 mg/ml |
| Conjugation | Alexa Fluor® 488 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| ICC | 1:500 | Hu |
| IF | 1:500 | Hu, Ms, Rt |
| ICFCM | 1:200 | Hu, Ms, Rt |
Background
Beta III tubulin (TUBB3) is a subtype of the beta-tubulin family, encoded by the TUBB3 gene, and plays a critical role in the development and function of the mammalian nervous system. As a neuron-specific cytoskeletal protein, it is highly expressed in the cell bodies, axons, and growth cones of embryonic and early postnatal neurons. Beyond participating in microtubule polymerization and stabilization, as well as maintaining neuronal morphology and polarity, its highly dynamic properties facilitate axon growth, guidance, and synaptic plasticity. Due to its stable and sustained expression in both immature and mature neurons, Beta III tubulin is widely used as a specific marker of the neuronal lineage in studies of neural development, neurotoxicity testing, and tumor classification (e.g., distinguishing neuroblastoma and neuroendocrine tumors).
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Picture
FC
Immunocytochemistry
ICC shows positive staining in SH-SY5Y cells. Anti-Beta III tubulin (Alexa Fluor® 488 Conjugate) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C.The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
Immunofluorescence
IF shows positive staining in paraffin-embedded human cerebellum. Anti-Beta III tubulin (Alexa Fluor® 488 Conjugate) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded mouse cerebellum. Anti-Beta III tubulin (Alexa Fluor® 488 Conjugate) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat cerebellum. Anti-Beta III tubulin (Alexa Fluor® 488 Conjugate) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
