WB result of Beta Actin Recombinant Mouse mAb
Primary antibody: Beta Actin Recombinant Mouse mAb at 1/40000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HEK-293 whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Lane 4: K562 whole cell lysate 20 µg
Lane 5: Jurkat whole cell lysate 20 µg
Secondary antibody: Goat Anti- mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 42 kDa
Observed MW: 42 kDa
Beta Actin Recombinant Mouse mAb (S-5033)
Beta Actin Recombinant Mouse mAb (S-5033)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | Beta Actin |
| Synonyms | Actin, cytoplasmic 1; ACTB |
| Location | Cytoplasm, Cytoskeleton, Nucleus |
| Accession | P60709 |
| Clone Number | S-5033 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2b |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | HeLa, HEK-293, HepG2, K562, Jurkat, NIH/3T3, 4T1, mouse brain, mouse colon, C6, PC-12, rat brain, rat colon, COS-7 |
| Purification | Protein A |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:10000-1:100000 | Hu, Ms, Rt, Mk |
| IHC-P | 1:1000 | Hu, Ms, Rt |
| ICC | 1:500 | Hu, Ms |
Background
Beta-actin is a fundamental component of the microfilament system within the cytoskeleton and is a highly conserved member of the actin protein family. Present in almost all eukaryotic cells, it exists in dynamic equilibrium between its monomeric (G-actin) and polymeric (F-actin) states. By polymerizing into helical microfilaments, it provides mechanical support and builds a network that governs intracellular transport, cell migration, division, and morphological maintenance. As a representative cytoplasmic actin, beta-actin not only mediates cell-matrix adhesion (e.g., focal adhesions) and cell-cell connections but also plays unconventional roles in transcriptional regulation and signal transduction by interacting with various proteins. Due to its extremely high and consistent expression levels across most tissues and cell types, the gene encoding beta-actin (ACTB) is widely used as a loading control or housekeeping gene reference in molecular biology experiments such as Western blot and qRT-PCR.
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Western Blot
WB result of Beta Actin Recombinant Mouse mAb
Primary antibody: Beta Actin Recombinant Mouse mAb at 1/40000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: 4T1 whole cell lysate 20 µg
Lane 3: mouse brain lysate 20 µg
Lane 4: mouse colon lysate 20 µg
Secondary antibody: Goat Anti- mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 42 kDa
Observed MW: 42 kDa
WB result of Beta Actin Recombinant Mouse mAb
Primary antibody: Beta Actin Recombinant Mouse mAb at 1/40000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: PC-12 whole cell lysate 20 µg
Lane 3: rat brain lysate 20 µg
Lane 4: rat colon lysate 20 µg
Secondary antibody: Goat Anti- mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 42 kDa
Observed MW: 42 kDa
WB result of Beta Actin Recombinant Mouse mAb
Primary antibody: Beta Actin Recombinant Mouse mAb at 1/40000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 42 kDa
Observed MW: 42 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-Beta Actin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human heart. Anti-Beta Actin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostate. Anti-Beta Actin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human skeletal muscle. Anti-Beta Actin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-Beta Actin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach cancer. Anti-Beta Actin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse heart. Anti-Beta Actin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat colon. Anti-Beta Actin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti- Beta Actin antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in NIH/3T3 cells. Anti- Beta Actin antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
