WB result of BCRP/ABCG2 Recombinant Rabbit mAb
Primary antibody: BCRP/ABCG2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled A204 whole cell lysate 20 µg
Lane 2: unboiled A549 whole cell lysate 20 µg
Lane 3: unboiled PANC-1 whole cell lysate 20 µg
Negative control: unboiled A204 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 72 kDa
Observed MW: 80 kDa
BCRP/ABCG2 Recombinant Rabbit mAb (S-2365-67)
BCRP/ABCG2 Recombinant Rabbit mAb (S-2365-67)
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | BCRP/ABCG2 |
| Synonyms | Broad substrate specificity ATP-binding cassette transporter ABCG2; ATP-binding cassette sub-family G member 2; Breast cancer resistance protein; CDw338; Mitoxantrone resistance-associated protein; Placenta-specific ATP-binding cassette transporter; Urate exporter; CD338; ABCP; BCRP1; MXR |
| Immunogen | Recombinant Protein |
| Location | Mitochondrion, Cell membrane |
| Accession | Q9UNQ0 |
| Clone Number | S-2365-67 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | A549, PANC-1, mouse liver, rat liver |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:2000 | Hu, Ms, Rt |
Background
The breast cancer resistance protein (BCRP/ABCG2), encoded by the ABCG2 gene, is a 655-amino-acid, 72–75 kDa half-transporter (a member of the ABC transporter superfamily G subfamily) that forms homodimers or oligomers to function as an ATP-dependent efflux pump actively transporting a broad range of substrates—including anticancer drugs (e.g., mitoxantrone, topotecan), endogenous compounds (e.g., estrone-3-sulfate, uric acid), and xenobiotics—across apical membranes of tissues such as the placenta, intestine, liver, kidney proximal tubules, and brain microvascular endothelium, thereby contributing to drug absorption, distribution, and elimination, protecting against xenobiotic exposure, and conferring multidrug resistance in cancer, particularly acute myeloid leukemia.
Picture
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Western Blot
WB result of BCRP/ABCG2 Recombinant Rabbit mAb
Primary antibody: BCRP/ABCG2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled mouse liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 72 kDa
Observed MW: 80 kDa
WB result of BCRP/ABCG2 Recombinant Rabbit mAb
Primary antibody: BCRP/ABCG2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled rat liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 72 kDa
Observed MW: 80 kDa
