BAP1 Recombinant Rabbit mAb (S-6199)

Catalog Number: S0B60524 Application: WB, IHC-P Reactivity: Hu, Ms, Rt Conjugation: Unconjugated Brand: Starter

Price:

$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	BAP1
Location	Cytoplasm, Nucleus
Accession	Q92560
Clone Number	S-6199
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu, Ms, Rt
Positive Sample	HeLa, PC-3, HepG2, A375, MDA-MB-231, A549, MOLT-4
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Hu, Ms, Rt
IHC-P	1:500	Hu, Ms, Rt

Background

BAP1 (BRCA1-associated protein 1) is an important tumor suppressor gene whose encoded protein belongs to the ubiquitin carboxyl-terminal hydrolase family. Its main function is to act as a deubiquitinating enzyme that removes ubiquitin molecules from substrates such as histone H2A, thereby participating in the regulation of gene expression, the cell cycle, DNA damage repair, and cell death, among other key cellular processes. BAP1 typically forms a multi-protein complex (PR-DUB) with proteins such as ASXL1/2, and through deubiquitination of H2A, it finely regulates chromatin status and transcriptional activity, thereby exerting its tumor-suppressive effects. When BAP1 acquires germline mutations or loss of function, it significantly increases an individual's risk of developing various tumors, including uveal melanoma, malignant mesothelioma, and clear cell renal cell carcinoma. This phenomenon is known as "BAP1 tumor predisposition syndrome."

Picture

Western Blot

WB result of BAP1 Recombinant Rabbit mAb
Primary antibody: BAP1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: PC-3 whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Lane 4: A375 whole cell lysate 20 µg
Lane 5: MDA-MB-231 whole cell lysate 20 µg
Lane 6: A549 whole cell lysate 20 µg
Lane 7: MOLT-4 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 80 kDa
Observed MW: 100 kDa
This blot was developed with high sensitivity substrate

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human testis. Anti-BAP1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human breast cancer. Anti-BAP1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human colon cancer. Anti-BAP1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human lung cancer. Anti-BAP1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse cerebrum. Anti-BAP1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat cerebrum. Anti-BAP1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.