WB result of Bak Recombinant Rabbit mAb
Primary antibody: Bak Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: THP-1 whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Lane 4: HT-29 whole cell lysate 20 µg
Lane 5: Molt-4 whole cell lysate 20 µg
Lane 6: RPMI-8226 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 23 kDa
Observed MW: 25 kDa
Bak Recombinant Rabbit mAb (S-2990-55)
Bak Recombinant Rabbit mAb (S-2990-55)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Bak |
| Synonyms | Bcl-2 homologous antagonist/killer; Apoptosis regulator BAK; Bcl-2-like protein 7 (Bcl2-L-7); BAK; BCL2L7; CDN1; BAK1 |
| Immunogen | Synthetic Peptide |
| Location | Mitochondrion |
| Accession | Q16611 |
| Clone Number | S-2990-55 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | HeLa, THP-1, Jurkat, HT-29, Molt-4, RPMI-8226, NIH/3T3, A20, rat spleen, COS-7 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt, Mk |
Background
Bak is a pro-apoptotic member of the Bcl-2 protein family that localizes to the mitochondrial outer membrane, where it functions as a key executor of apoptosis; upon activation by BH3-only proteins or other stimuli, Bak undergoes conformational changes, oligomerizes, and forms pores in the membrane, leading to the release of cytochrome c and other apoptogenic factors into the cytosol to trigger programmed cell death.
Picture
Picture
Western Blot
WB result of Bak Recombinant Rabbit mAb
Primary antibody: Bak Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: A20 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 23 kDa
Observed MW: 25 kDa
WB result of Bak Recombinant Rabbit mAb
Primary antibody: Bak Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat spleen lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 23 kDa
Observed MW: 25 kDa
WB result of Bak Recombinant Rabbit mAb
Primary antibody: Bak Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 23 kDa
Observed MW: 25 kDa
