1 1

BAFF&APRIL Heterotrimer His Tag Protein, Human

BAFF&APRIL Heterotrimer His Tag Protein, Human

Catalog Number: UA011338 Brand: UA BIOSCIENCE
Share:
Price:
$235 USD
$235 USD
Size:

For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details

Product Details

Product Specification


Species Human
Synonyms BAFF&APRIL
Accession Q9Y275、O75888
Amino Acid Sequence

Glu140-Leu285&Gln111-Leu250 with His Tag at the N-Terminus
Expression System HEK293
Molecular Weight

45-65kDa (Reducing)
Purity ＞95% by SDS-PAGE & > 95% SEC-HPLC
Conjugation Unconjugated
Tag His Tag
Physical Appearance Lyophilized powder
Storage Buffer

PBS, PH7.4, 5% trehalose
Reconstitution

Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage

· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
Reference

1.Wiggins CD, Sangés Ametllé M, Wu C, Boswell KL, Watkins TA, Koup RA, Lauffenburger DA. Multivariate analysis of glycogenes reveals coordinated regulation of immunoglobulin glycosylation in an immortalized human B cell system. bioRxiv [Preprint]. 2025 Oct 2:2025.09.30.679651.
2.Rybka G, Dziedzic R, Węglarczyk K, Milewski M, Siwiec-Koźlik A, Dziedzina S, Sanak M, Musiał J, Siedlar M, Korkosz M, Kosałka-Węgiel J. Serum levels of APRIL, BAFF, and IL-10 in systemic lupus erythematosus have limited utility as biomarkers for disease activity or flare prediction. Clin Rheumatol. 2025 Nov 16.

Background

BAFF (B-cell activating factor) and APRIL (a proliferation-inducing ligand) are members of the tumor necrosis factor (TNF) superfamily. They typically exist as homotrimers but can also form heterotrimers. Secreted by activated myeloid cells, they exert immunomodulatory effects by regulating B-cell differentiation, proliferation, survival, and effector functions. Clinically, dysregulated expression of the BAFF/APRIL system is closely associated with autoimmune diseases, particularly with significantly elevated levels observed in patients with systemic lupus erythematosus (SLE). The detection of circulating heterotrimers in the serum of patients with systemic immune rheumatic diseases suggests that these heterotrimers may represent a novel therapeutic target for rheumatic and autoimmune disorders.

Picture

SDS-PAGE

1μg (R: reducing condition, N:non-reducing condition).

SEC-HPLC

The purity of BAFF&APRIL Heterotrimer His Tag Protein, Human is more than 95% determined by SEC-HPLC.

ELISA

Immobilized BAFF&APRIL Heterotrimer His Tag Protein, Human (Cat. No. UA011338) at 2.0μg/mL (100μL/well) can bind Biotinylated BAFFR/TNFRSF13C Fc&Avi Tag Protein, Human (Cat. No. UA011346) with EC50 of 24.45-41.71 ng/mL.

Immobilized BAFF&APRIL Heterotrimer His Tag Protein, Human (Cat. No. UA011338) at 2.0μg/mL (100μL/well) can bind TACI/CD267/TNFRSF13B Fc Chimera Protein, Human (Cat. No. UA011118) with EC50 of 1.65-2.67 ng/mL.