mIHC shows positive staining in paraffin-embedded human tonsil (A). Anti- Vimentin Rabbit mAb (S0B2254) was used at 1/1000 dilution (White, B), Anti-CD4 Rabbit mAb (S0B2179) was used at 1/500 dilution (Yellow, C), Anti-CD8α Mouse mAb (S0B2148) was used at 1/1000 dilution (Orange, D), Anti- Granzyme B Rabbit mAb (S0B0359) was used at 1/200 dilution (Green, E), and Anti- B7-H3 Rabbit mAb (S0B2244) was used at 1/1000 dilution (Red, F), followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with mIHC staining protocol.
Product Details
Product Details
Product Specification
Antibody Type | Recombinant mAb |
Application | mIHC |
Reactivity | Hu |
Conjugation | Unconjugated |
Physical Appearance | Liquid |
Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
application | dilution | species |
mIHC | 1:200-1:1000 |
Background
B7-H3, Vimentin, CD8α, CD4, Granzyme B Antibody Panel contains 5 recombinant monoclonal antibodies against PD-L1, CK-Pan, CD8α, CD4, CD163. They are provided as a sampler panel. Panel contains: - B7-H3 Recombinant Rabbit mAb (SDT-249-26) S0B2244 - Vimentin Recombinant Rabbit mAb (SDT-029-120) S0B2254 - CD8α Recombinant Mouse mAb (SDT-126-8) S0B2148 - CD4 Recombinant Rabbit mAb (SDT-R163) S0B2179 - Granzyme B Recombinant Rabbit mAb (S-357-54) S0B0359
Picture
Picture
Contents & Protocol
Multiple Immunohistochemistry
