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B7-H2/ICOSLG Recombinant Rabbit mAb (S-3306-12)

B7-H2/ICOSLG Recombinant Rabbit mAb (S-3306-12)

Catalog Number: S0B6596 Application: WB, IHC-P Reactivity: Human Conjugation: Unconjugated Brand: Starter
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Product Details

Product Specification


Host Rabbit
Antigen CD275
Synonyms ICOS ligand; B7 homolog 2 (B7-H2); B7-like protein Gl50; B7-related protein 1 (B7RP-1); B7H2; B7RP1; ICOSL; KIAA0653; ICOSLG
Immunogen Synthetic Peptide
Location Cell membrane
Accession O75144
Clone Number S-3306-12
Antibody Type Recombinant mAb
Isotype IgG
Application WB, IHC-P
Reactivity Hu
Positive Sample Raji, Daudi, HL-60, MCF7
Purification Protein A
Concentration 0.5 mg/ml
Conjugation Unconjugated
Physical Appearance Liquid
Storage Buffer

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage

12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution


application dilution species
WB 1:500-1:1000 Hu
IHC-P 1:250 Hu

Background

CD275, also called ICOS ligand (ICOSL) or B7-H2, is a 40–60 kDa glycosylated type-I transmembrane protein encoded by the ICOSLG gene on chromosome 21 and classified within the B7 and immunoglobulin superfamilies; it is expressed on B cells, dendritic cells, macrophages, monocytes, endothelial cells and various non-hematopoietic tissues, can be rapidly up-regulated by IFN-γ, TNF-α or LPS, and serves as the indispensable ligand for the inducible costimulator ICOS on activated T cells, thereby delivering a critical positive co-stimulatory signal that drives T-cell activation, proliferation, Th2 cytokine secretion and the generation of follicular helper T cells and germinal centers while simultaneously promoting B-cell differentiation into plasma cells and antibody production during secondary immune responses, and plays additional roles in reactivating effector/memory T cells on inflamed endothelium to control tissue entry and in skewing maternal immunity toward a protective Th2 phenotype during pregnancy; its dysregulation contributes to infection, hypersensitivity, autoimmune diseases, transplantation immunity and tumor immunity, and alternative splicing yields multiple isoforms with distinct tissue distributions and functions.

Picture

Western Blot

WB result of B7-H2/ICOSLG Recombinant Rabbit mAb
Primary antibody: B7-H2/ICOSLG Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Jurkat whole cell lysate 20 µg
Lane 2: Raji whole cell lysate 20 µg
Lane 3: Daudi whole cell lysate 20 µg
Lane 4: HL-60 whole cell lysate 20 µg
Lane 5: MCF7 whole cell lysate 20 µg
Low expression control: Jurkat whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 33 kDa
Observed MW: 40-80 kDa
This blot was developed with high sensitivity substrate

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human tonsil. Anti- B7-H2/ICOSLG antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human breast cancer. Anti- B7-H2/ICOSLG antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti- B7-H2/ICOSLG antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.