1 1

AXL Recombinant Rabbit mAb (S-2199-80)

AXL Recombinant Rabbit mAb (S-2199-80)

Catalog Number: S0B6609 Application: WB, IHC-P, ICC Reactivity: Human Conjugation: Unconjugated Brand: Starter
Share:
Price:
$100 USD
$100 USD
Size:

For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details

Product Details

Product Specification


Host Rabbit
Antigen AXL
Synonyms Tyrosine-protein kinase receptor UFO; AXL oncogene; UFO
Immunogen Recombinant Protein
Location Cell membrane
Accession P30530
Clone Number S-2199-80
Antibody Type Recombinant mAb
Isotype IgG
Application WB, IHC-P, ICC
Reactivity Hu
Positive Sample HeLa, HUVEC, COS-7
Purification Protein A
Concentration 0.5 mg/ml
Conjugation Unconjugated
Physical Appearance Liquid
Storage Buffer

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage

12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution


application dilution species
WB 1:1000 Hu, Mk
IHC-P 1:100 Hu
ICC 1:500 Hu

Background

AXL protein is a receptor tyrosine kinase and a member of the Tyro3-Axl-Mer (TAM) subfamily, characterized by an extracellular domain with two immunoglobulin-like motifs and two fibronectin type-III motifs. It transduces signals from the extracellular matrix into the cytoplasm by binding to the vitamin K-dependent protein Gas6. This binding induces AXL dimerization and autophosphorylation, activating downstream molecules like PI3-kinase subunits, GRB2, and PLCG1, which in turn triggers AKT kinase activation. AXL is involved in various cellular functions, including cell survival, proliferation, migration, and differentiation. It also plays a role in immune modulation by inhibiting Toll-like receptor-mediated innate immune responses. Additionally, AXL serves as a host cell receptor for several viruses, such as Marburg, Ebola, Lassa, and potentially SARS-CoV-2.

Picture

Western Blot

WB result of AXL Recombinant Rabbit mAb
Primary antibody: AXL Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Jurkat whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: HUVEC whole cell lysate 20 µg
Negative control: Jurkat whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 98 kDa
Observed MW: 130 kDa

WB result of AXL Recombinant Rabbit mAb
Primary antibody: AXL Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 98 kDa
Observed MW: 125 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human colon. Anti-AXL antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human liver. Anti-AXL antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human colon cancer. Anti-AXL antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Immunocytochemistry

ICC shows positive staining in HeLa cells (top panel) and negative staining in Jurkat cells (below panel). Anti-AXL antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).