Aurora A Recombinant Rabbit mAb (S-5355)

Catalog Number: S0B60347 Application: WB, IHC-P Reactivity: Hu Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	Aurora A
Synonyms	Aurora kinase A; Aurora 2; Aurora/IPL1-related kinase 1 (ARK-1; Aurora-related kinase 1); Breast tumor-amplified kinase; Ipl1- and aurora-related kinase 1; Serine/threonine-protein kinase 15; AIK; AIRK1; ARK1; AURA; AYK1; BTAK; IAK1; STK15 s; STK6; AURKA
Location	Cytoplasm, Cytoskeleton
Accession	O14965
Clone Number	S-5355
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000-1:5000	Hu
IHC-P	1:500	Hu

Background

Aurora A is a serine/threonine kinase that plays a pivotal role in regulating multiple stages of mitosis, including centrosome maturation, spindle assembly, chromosome alignment, and cytokinesis, by phosphorylating key substrates such as TPX2, TACC3, and histone H3 to ensure accurate cell division. Overexpression or amplification of the AURKA gene encoding Aurora A is frequently observed in various human cancers, where it correlates with chromosomal instability, tumor aggressiveness, and poor prognosis, making it an attractive therapeutic target; consequently, numerous small-molecule inhibitors like alisertib have been developed and evaluated in clinical trials, although challenges remain regarding selectivity, resistance mechanisms, and optimal combination strategies to effectively exploit Aurora A dependency in malignancies while minimizing toxicity to normal proliferating tissues.

Picture

Western Blot

WB result of Aurora A Recombinant Rabbit mAb
Primary antibody: Aurora A Recombinant Rabbit mAb at 1/1000 dilution 4℃ overnight
Lane 1: untreated HT-29 whole cell lysate 20 µg
Lane 2: HT-29 treated with nocodazole (100ng/mL, 16 hours) whole cell lysate 20 µg
Lane 3: untreated HeLa whole cell lysate 20 µg
Lane 4: HeLa treated with nocodazole (100ng/mL, 16 hours) whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 46 kDa
Observed MW: 48 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human tonsil. Anti-Aurora A antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-Aurora A antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human lung cancer. Anti-Aurora A antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.