WB result of ATRIP Recombinant Rabbit mAb
Primary antibody: ATRIP Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-3 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: MCF7 whole cell lysate 20 µg
Lane 4: HepG2 whole cell lysate 20 µg
Lane 5: LNCaP whole cell lysate 20 µg
Lane 6: OVCAR-3 whole cell lysate 20 µg
Lane 7: HEK-293 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 86 kDa
Observed MW: 90 kDa
ATRIP Recombinant Rabbit mAb (S-1275-48)
ATRIP Recombinant Rabbit mAb (S-1275-48)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | ATRIP |
| Synonyms | ATR-interacting protein; ATM and Rad3-related-interacting protein; AGS1 |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | Q8WXE1 |
| Clone Number | S-1275-48 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IP |
| Reactivity | Hu |
| Positive Sample | PC-3, HeLa, MCF7, HepG2, LNCaP, OVCAR-3, HEK-293 |
| Predicted Reactivity | Mk, Ms |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IP | 1:50 | Hu |
Background
ATRIP stands for ATR-interacting protein. It works together with ATR (ataxia-telangiectasia mutated and Rad3-related) as a crucial component in the cell cycle checkpoint signaling pathway. Both ATR and ATRIP localize to intranuclear foci following DNA damage or replication inhibition. They are essential for the viability of human somatic cells and are interdependent in the cell cycle checkpoint signaling pathways. Furthermore, ATRIP plays a significant role in the DNA damage response. Together with ATR, it phosphorylates and transmits damage signals to effectors, initiating the repair process.
Picture
Picture
Western Blot
IP
ATRIP Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating ATRIP in 0.4 mg HeLa whole cell lysate.
Western blot was performed on the immunoprecipitate using ATRIP Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: HeLa whole cell lysate 20 µg (Input)
Lane 2: ATRIP Rabbit mAb IP in HeLa whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
Predicted MW: 86 kDa
Observed MW: 90 kDa
