WB result of ATPIF1 Recombinant Rabbit mAb
Primary antibody: ATPIF1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: K-562 whole cell lysate 20 µg
Lane 3: MCF7 whole cell lysate 20 µg
Lane 4: HEK-293 whole cell lysate 20 µg
Lane 5: HCT 116 whole cell lysate 20 µg
Lane 6: HT-29 whole cell lysate 20 µg
Lane 7: Capan-1 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 12 kDa
Observed MW: 13 kDa
ATPIF1 Recombinant Rabbit mAb (S-3849-49)
ATPIF1 Recombinant Rabbit mAb (S-3849-49)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | ATPIF1 |
| Synonyms | ATPase inhibitor, mitochondrial; ATP synthase F1 subunit epsilon; Inhibitor of F(1)F(o)-ATPase (IF(1); IF1); ATPI; ATP5IF1 |
| Immunogen | Recombinant Protein |
| Location | Mitochondrion |
| Accession | Q9UII2 |
| Clone Number | S-3849-49 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu |
| Positive Sample | HeLa, K562, MCF7, HEK293, HCT 116, HT-29, Capan-1 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:10000 | Hu |
| IHC-P | 1:2000 | Hu |
| ICC | 1:500 | Hu |
Background
ATPIF1 is a small regulatory protein primarily localized in the mitochondrial matrix, composed of 106 amino acids, with its core function serving as an "intelligent brake" for mitochondrial ATP synthase. Its most important physiological role is to prevent ATP synthase from hydrolyzing ATP in reverse when the proton motive force declines, thereby avoiding wasteful consumption of precious ATP and providing an energy-saving protective effect. The activation of this "brake" function is mainly regulated by the pH of the mitochondrial matrix—when the environment acidifies (pH decreases), ATPIF1 is activated and binds to ATP synthase, inhibiting its hydrolytic activity. Interestingly, the functions of ATPIF1 extend far beyond this; it can also participate in shaping mitochondrial cristae structure by promoting the formation of ATP synthase dimers or tetramers. In cancer cells, overexpression of ATPIF1 suppresses mitochondrial ATP synthesis, forcing cells to shift toward glycolysis (the Warburg effect), a metabolic reprogramming that is believed to favor tumor growth and survival. In diseases such as heart failure, however, abnormal upregulation of ATPIF1 may instead impair cardiomyocyte energy metabolism and contractile function, exacerbating the pathological condition.
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Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-ATPIF1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-ATPIF1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-ATPIF1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach. Anti-ATPIF1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-ATPIF1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-ATPIF1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-ATPIF1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti-ATPIF1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti- ATPIF1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
