WB result of ATP5A Rabbit pAb
Primary antibody: ATP5A Rabbit pAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Lane 4: A549 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 60 kDa
Observed MW: 50 kDa
ATP5A Rabbit Polyclonal Antibody
ATP5A Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | ATP5A |
| Synonyms | ATP synthase subunit alpha, mitochondrial; ATP synthase F1 subunit alpha; ATP5F1A; ATP5A1; ATP5AL2; ATPM |
| Immunogen | Synthetic Peptide |
| Location | Mitochondrion, Cell membrane |
| Accession | P25705 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, IHC-P, ICC, IP |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, HepG2, Jurkat, A549, Neuro-2a, PC-12 |
| Predicted Reactivity | Or, Pg, Bv, Fr, Ys, Nm |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IP | 1:50 | Hu |
| IHC-P | 1:200 | Hu, Ms, Rt |
| ICC | 1:500 | Hu |
Background
ATP5A is the alpha subunit of the mitochondrial ATP synthase. The ATP synthase has two main functional domains: the catalytic core known as F1 and the membrane-embedded proton channel called Fo. The F1 part consists of three alpha subunits (ATP5A1) and three beta subunits (ATP5B), forming a hexamer with six ADP/ATP binding sites. Only the beta subunits are catalytically active in ATP synthesis/hydrolysis, while the alpha subunits are catalytically inactive. ATP5A1 is expressed in various tissues, including the heart and kidneys, and has broad species reactivity, such as in mice, rats, cows, humans, and fruit flies. Beyond its role in energy metabolism, studies suggest that ATP5A1 may also be involved in the post-transcriptional regulation of cancer-related genes and is associated with the development of various diseases.
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Western Blot
WB result of ATP5A Rabbit pAb
Primary antibody: ATP5A Rabbit pAb at 1/1000 dilution
Lane 1: Neuro-2a whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 60 kDa
Observed MW: 50 kDa
WB result of ATP5A Rabbit pAb
Primary antibody: ATP5A Rabbit pAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 60 kDa
Observed MW: 50 kDa
IP
ATP5A Rabbit pAb at 1/50 dilution (1 µg) immunoprecipitating ATP5A in 0.4 mg HeLa whole cell lysate.
Western blot was performed on the immunoprecipitate using ATP5A Rabbit pAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: HeLa whole cell lysate 20 µg (Input)
Lane 2: ATP5A Rabbit pAb IP in HeLa whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
Predicted MW: 60 kDa
Observed MW: 50 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human kidney. Anti-ATP5A antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-ATP5A antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-ATP5A antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human skeletal muscle. Anti-ATP5A antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-ATP5A antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti-ATP5A antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in MCF7 cells. Anti-ATP5A antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
