IHC shows positive staining in paraffin-embedded human stomach. Anti-ATP4B antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
ATP4B Recombinant Rabbit mAb (SDT-2445-64)
ATP4B Recombinant Rabbit mAb (SDT-2445-64)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | ATP4B |
| Synonyms | Potassium-transporting ATPase subunit beta; Gastric H(+)/K(+) ATPase subunit beta; Proton pump beta chain |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane |
| Accession | P51164 |
| Clone Number | SDT-2445-64 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | IHC-P, IF |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.25 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| IHC-P | 1:1000-1:2000 | Hu |
| IF | 1:250-1:500 | Hu |
Background
ATP4B (ATPase H+/K+ transporting subunit beta) is a 291-amino-acid, heavily glycosylated transmembrane protein that forms the regulatory β-subunit of the heterodimeric gastric H+/K+-ATPase proton pump, which exchanges cytoplasmic H+ for extracellular K+ across the apical membrane of parietal cells to drive gastric acid secretion; it stabilizes and targets the catalytic α-subunit to the membrane, acts as a conformational ratchet that locks the pump in its outward-facing E2 state, and contributes to epithelial cell polarity and adhesion through an immunoglobulin-like C-terminal ectodomain .
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Immunohistochemistry
Negative control: IHC shows negative staining in paraffin-embedded human gastric cancer. Anti-ATP4B antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded human stomach. Anti-ATP4B antibody was used at 1/250 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
