ATP1B1 Rabbit Polyclonal Antibody

Catalog Number: S0B6988 Application: WB, IHC-P Reactivity: Hu, Ms, Rt Conjugation: Unconjugated Brand: Starter

Price:

$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	ATP1B1
Synonyms	Sodium/potassium-transporting ATPase subunit beta-1; ATP1B
Immunogen	Synthetic Peptide
Location	Cell membrane
Accession	P05026
Antibody Type	Polyclonal antibody
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu, Ms, Rt
Purification	Immunogen Affinity
Concentration	2 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Hu, Ms, Rt
IHC-P	1:250	Hu, Ms

Background

ATP1B1, also known as the sodium/potassium-transporting ATPase subunit beta-1, is an essential membrane glycoprotein that serves as the beta subunit of the Na⁺/K⁺-ATPase enzyme complex, which is fundamental for maintaining electrochemical gradients across the plasma membrane of most animal cells. This protein plays a critical role in the structural stability and functional maturation of the alpha catalytic subunit, facilitating its correct folding, trafficking to the cell surface, and protection from proteolytic degradation, thereby enabling the active transport of three sodium ions out of the cell and two potassium ions into the cell against their concentration gradients using energy derived from ATP hydrolysis. Beyond its housekeeping function in regulating cell volume, resting membrane potential, and secondary active transport systems, ATP1B1 is increasingly recognized for its involvement in cell adhesion and signal transduction pathways, acting as a modulator in various physiological processes and pathological conditions, including neurological disorders, cardiovascular diseases, and cancer progression, where its expression levels often correlate with tumor suppression or metastatic potential depending on the cellular context.

Picture

Western Blot

WB result of ATP1B1 Rabbit pAb
Primary antibody: ATP1B1 Rabbit pAb at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Lane 2: mouse kidney lysate 20 µg
Lane 3: mouse heart lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 35 kDa
Observed MW: 40-55 kDa

WB result of ATP1B1 Rabbit pAb
Primary antibody: ATP1B1 Rabbit pAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Lane 2: rat kidney lysate 20 µg
Lane 3: rat heart lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 35 kDa
Observed MW: 40-55 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-ATP1B1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human kidney. Anti-ATP1B1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human colon cancer. Anti-ATP1B1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse kidney. Anti-ATP1B1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.