ATP Citrate Lyase Recombinant Rabbit mAb (S-5036)

Catalog Number: S0B6902 Application: WB, IHC-P, ICC Reactivity: Hu, Ms, Rt, Mk Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25µl 100µl

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Product Details

Product Specification

Host	Rabbit
Antigen	ATP citrate lyase
Synonyms	ATP-citrate synthase; ATP-citrate (pro-S-)-lyase (ACL); Citrate cleavage enzyme; ACLY
Location	Cytoplasm
Accession	P53396
Clone Number	S-5036
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P, ICC
Reactivity	Hu, Ms, Rt, Mk
Positive Sample	HeLa, NIH/3T3, mouse lung, C6, rat lung, COS-7
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Hu, Ms, Rt, Mk
IHC-P	1:2000	Hu, Ms, Rt
ICC	1:500	Hu

Background

ACLY, also known as ATP citrate lyase, is a crucial enzyme involved in cellular metabolism. It catalyzes the conversion of citrate and ATP to oxaloacetate, acetyl-CoA, and ADP, playing a key role in linking glycolysis and lipid metabolism. ACLY is predominantly located in the cytoplasm and is essential for the production of acetyl-CoA, which serves as a vital precursor for fatty acid synthesis and cholesterol biosynthesis. Its activity is tightly regulated by various factors, including insulin and AMPK, reflecting its importance in maintaining metabolic homeostasis. Abnormal expression or activity of ACLY has been implicated in several diseases, such as cancer and metabolic disorders, making it an attractive target for therapeutic interventions.

Picture

Western Blot

WB result of ATP Citrate Lyase Recombinant Rabbit mAb
Primary antibody: ATP Citrate Lyase Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 121 kDa
Observed MW: 121 kDa

WB result of ATP Citrate Lyase Recombinant Rabbit mAb
Primary antibody: ATP Citrate Lyase Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse lung lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 121 kDa
Observed MW: 121 kDa

WB result of ATP Citrate Lyase Recombinant Rabbit mAb
Primary antibody: ATP Citrate Lyase Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat lung lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 121 kDa
Observed MW: 121 kDa

WB result of ATP Citrate Lyase Recombinant Rabbit mAb
Primary antibody: ATP Citrate Lyase Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 121 kDa
Observed MW: 121 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human heart. Anti- ATP citrate lyase antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human lung cancer. Anti- ATP citrate lyase antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human renal cell carcinoma. Anti- ATP citrate lyase antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse kidney. Anti- ATP citrate lyase antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat testis. Anti- ATP citrate lyase antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Immunocytochemistry

ICC shows positive staining in HeLa cells. Anti- ATP Citrate Lyase antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).