WB result of Atg3 Recombinant Rabbit mAb
Primary antibody: Atg3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HL-60 whole cell lysate 20 µg
Lane 2: K562 whole cell lysate 20 µg
Lane 3: THP-1 whole cell lysate 20 µg
Lane 4: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 36 kDa
Observed MW: 38 kDa
Atg3 Recombinant Rabbit mAb (S-2168-20)
Atg3 Recombinant Rabbit mAb (S-2168-20)
Price:
Regular price
$100 USD
Regular price
Sale price
$100 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Atg3 |
| Synonyms | Ubiquitin-like-conjugating enzyme ATG3; Autophagy-related protein 3 (APG3-like; hApg3); Protein PC3-96; APG3; APG3L; ATG3 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm |
| Accession | Q9NT62 |
| Clone Number | S-2168-20 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | HL-60, K562, THP-1, HeLa, A20, mouse kidney, PC-12, COS-7 |
| Predicted Reactivity | Bv |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt, Mk |
| IHC-P | 1:200 | Hu |
| ICC | 1:100 | Hu |
Background
Atg3 is an E2-like enzyme essential for autophagosome biogenesis: its C-terminal catalytic cysteine forms a thioester bond with the C-terminal glycine of Atg8-family proteins (LC3/GABARAPs) in an ATP-dependent manner, and subsequently, with the E3-like Atg12–Atg5-Atg16L1 complex, it covalently conjugates Atg8 to phosphatidylethanolamine on the expanding phagophore membrane, thereby driving membrane curvature, expansion, and closure into the double-membrane autophagosome that sequesters cytoplasmic cargo for lysosomal degradation.
Picture
Picture
Western Blot
WB result of Atg3 Recombinant Rabbit mAb
Primary antibody: Atg3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A20 whole cell lysate 20 µg
Lane 2: mouse kidney lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 36 kDa
Observed MW: 38 kDa
WB result of Atg3 Recombinant Rabbit mAb
Primary antibody: Atg3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 36 kDa
Observed MW: 38 kDa
WB result of Atg3 Recombinant Rabbit mAb
Primary antibody: Atg3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 36 kDa
Observed MW: 38 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human kidney. Anti-Atg3 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon. Anti-Atg3 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-Atg3 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti- Atg3 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
