WB result of ATF3 Recombinant Rabbit mAb
Primary antibody: ATF3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Daudi whole cell lysate 20 µg
Lane 2: HCT 116 whole cell lysate 20 µg
Lane 3: 293T whole cell lysate 20 µg
Negative control: Daudi whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 21 kDa
Observed MW: 26 kDa
ATF3 Recombinant Rabbit mAb (S-3904)
ATF3 Recombinant Rabbit mAb (S-3904)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | ATF3 |
| Synonyms | Cyclic AMP-dependent transcription factor ATF-3; cAMP-dependent transcription factor ATF-3; Activating transcription factor 3 |
| Location | Nucleus |
| Accession | P18847 |
| Clone Number | S-3904 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms |
| Positive Sample | HeLa, HepG2, HCT 116, 293T, LNCaP, RAW264.7 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms |
| IHC-P | 1:500 | Hu |
| ICC | 1:500 | Hu, Ms |
Background
ATF3 (Activating Transcription Factor 3) is a 181-amino-acid stress-inducible bZIP transcription factor that binds DNA as homo- or heterodimer, recognized by the consensus TGACGTCA, and acts as a dual-function transcriptional regulator—repressing or activating target genes involved in apoptosis, inflammation, cell-cycle checkpoints, metabolic reprogramming and oncogenesis—rapidily up-regulated in response to hypoxia, DNA damage, cytokines, ER stress or mechanical strain, frequently overexpressed in cancers where it promotes survival, metastasis and chemoresistance yet can also function as a tumor suppressor depending on cellular context, and is subject to tight control via MAPK, NF-κB, p53 and multiple feedback loops, making it a critical node in integrating stress signals and determining cell fate.
Picture
Picture
Western Blot
WB result of ATF3 Recombinant Rabbit mAb
Primary antibody: ATF3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated THP-1 whole cell lysate 20 µg
Lane 2: THP-1 treated with 80 nM Phorbol-myristate-acetate for 16 hours, then treated with 1 µg/ml LPS for 8 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 21 kDa
Observed MW: 26 kDa
WB result of ATF3 Recombinant Rabbit mAb
Primary antibody: ATF3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated RAW264.7 whole cell lysate 20 µg
Lane 2: RAW264.7 treated with 1 µg/ml LPS for 2 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 21 kDa
Observed MW: 26 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human placenta. Anti-ATF3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human Hodgkin’s lymphoma. Anti-ATF3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-ATF3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-ATF3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC analysis of THP-1 cells treated with Phorbol-myristate-acetate(80nM,16h), then LPS(1ug/ml,8h) (top panel) and untreated THP-1 cells (below panel). Anti- ATF3 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC analysis of RAW264.7 cells treated with LPS (1ug/ml 2h) (top panel) and untreated RAW264.7 cells (below panel). Anti- ATF3 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
