WB result of ATF1 Recombinant Rabbit mAb
Primary antibody: ATF1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: 293T whole cell lysate 20 µg
Lane 3: A431 whole cell lysate 20 µg
Lane 4: K562 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 29 kDa
Observed MW: 37 kDa
This blot was developed with high sensitivity substrate
ATF1 Recombinant Rabbit mAb (S-2883-124)
ATF1 Recombinant Rabbit mAb (S-2883-124)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | ATF1 |
| Synonyms | Cyclic AMP-dependent transcription factor ATF-1; cAMP-dependent transcription factor ATF-1; Activating transcription factor 1; TCR-ATF1; Atf1 |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | P81269 |
| Clone Number | S-2883-124 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu, Ms |
| Positive Sample | HeLa, 293T, A431, K562, RAW264.7 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms |
| ICC | 1:500 | Hu, Ms |
Background
ATF1 (activating transcription factor 1) is a 271-amino-acid basic leucine-zipper (bZIP) protein that forms homo- or heterodimers with c-Jun, CREB or other bZIP partners to bind the consensus cAMP response element 5′-TGACGTCA-3′, thereby functioning as a stimulus-inducible transcriptional activator in cAMP, MAPK, calcium and stress-signaling cascades that govern proliferation, survival, melanocyte differentiation, synaptic plasticity and memory; it is subject to phosphorylation-driven control (notably by PKA at Ser63 and by p38/MSK1 at Ser63/121), acetylation and ubiquitination, is frequently fused to either EWSR1 or FUS in Ewing sarcoma and angiomatoid fibrous histiocytoma through t(12;22) or t(12;16) chromosomal translocations that convert it into an aberrant transcriptional activator, and its locus at 12q13 is co-amplified with CDK4 in a variety of sarcomas, underscoring its dual role as both a tightly regulated physiological transcription factor and an oncogenic driver.
Picture
Picture
Western Blot
WB result of ATF1 Recombinant Rabbit mAb
Primary antibody: ATF1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: RAW264.7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 29 kDa
Observed MW: 37 kDa
This blot was developed with high sensitivity substrate
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti-ATF1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in RAW264.7 cells. Anti-ATF1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
