ATAD2 Recombinant Rabbit mAb (S-4145-63)

Catalog Number: S0B6987 Application: WB, IHC-P Reactivity: Hu, Ms, Rt, Mk Conjugation: Unconjugated Brand: Starter

Price:

$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	ATAD2
Synonyms	ATPase family AAA domain-containing protein 2
Immunogen	Synthetic Peptide
Location	Nucleus
Accession	Q6PL18
Clone Number	S-4145-63
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu, Ms, Rt, Mk
Positive Sample	HeLa, MCF7, K562, F9, C6, rat testis, COS-7
Purification	Protein A
Concentration	2 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Hu, Ms, Rt, Mk
IHC-P	1:100	Hu, Ms, Rt

Background

ATAD2 (ATPase Family AAA Domain Containing 2) is a nuclear oncoprotein that functions as a critical epigenetic reader and transcriptional co-regulator, primarily recognized for its ability to bind acetylated histones through its bromodomain and interact with various transcription factors via its AAA+ ATPase domain. Overexpressed in a wide array of malignancies—including lung, breast, prostate, and gastric cancers—ATAD2 promotes tumorigenesis by enhancing the expression of genes involved in cell proliferation, survival, and metastasis, often correlating with poor patient prognosis and reduced overall survival. Mechanistically, it facilitates chromatin remodeling and stabilizes protein complexes at promoter regions, thereby driving the transcriptional programs necessary for cancer cell growth, which has positioned ATAD2 as a promising therapeutic target and biomarker for early diagnosis and precision oncology strategies aiming to inhibit its bromodomain-mediated interactions.

Picture

Western Blot

WB result of ATAD2 Recombinant Rabbit mAb
Primary antibody: ATAD2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: MCF7 whole cell lysate 20 µg
Lane 3: K562 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 158 kDa
Observed MW: 180 kDa

WB result of ATAD2 Recombinant Rabbit mAb
Primary antibody: ATAD2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: F9 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 158 kDa
Observed MW: 180 kDa

WB result of ATAD2 Recombinant Rabbit mAb
Primary antibody: ATAD2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat testis lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 158 kDa
Observed MW: 180 kDa

WB result of ATAD2 Recombinant Rabbit mAb
Primary antibody: ATAD2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 158 kDa
Observed MW: 180 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human testis. Anti-ATAD2 antibody was used at 1/100 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human lung cancer. Anti-ATAD2 antibody was used at 1/100 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human gastirc cancer. Anti-ATAD2 antibody was used at 1/100 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded human skeletal muscle. Anti-ATAD2 antibody was used at 1/100 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse colon. Anti-ATAD2 antibody was used at 1/100 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse testis. Anti-ATAD2 antibody was used at 1/100 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded mouse skeletal muscle. Anti-ATAD2 antibody was used at 1/100 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat testis. Anti-ATAD2 antibody was used at 1/100 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded rat skeletal muscle. Anti-ATAD2 antibody was used at 1/100 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.