WB result of ARIH1 Recombinant Rabbit mAb
Primary antibody: ARIH1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: A431 whole cell lysate 20 µg
Lane 3: MCF7 whole cell lysate 20 µg
Lane 4: PC-3 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 64 kDa
Observed MW: 64 kDa
ARIH1 Recombinant Rabbit mAb (S-3166-64)
ARIH1 Recombinant Rabbit mAb (S-3166-64)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | ARIH1 |
| Synonyms | E3 ubiquitin-protein ligase ARIH1; H7-AP2; HHARI; Monocyte protein 6 (MOP-6); Protein ariadne-1 homolog (ARI-1); UbcH7-binding protein; ARI; MOP6; UBCH7BP |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | Q9Y4X5 |
| Clone Number | S-3166-64 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | HeLa, A431, MCF7, PC-3, NIH/3T3, C6, COS-7 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt, Mk |
| ICC | 1:500 | Hu, Ms |
Background
ARIH1 (Ariadne RBR E3 ubiquitin-protein ligase 1) is a 557-amino-acid RING-between-RING (RBR) E3 ligase that ubiquitinates multiple substrates—most notably DNA-PKcs, whose proteasomal degradation activates the cGAS-STING pathway to enhance anti-tumor immunity, while in colorectal cancer it instead promotes K63-linked ubiquitination of PHB1 to drive mitochondrial respiration and tumor progression—thereby acting as a context-dependent regulator of proliferation, DNA repair, epithelial-mesenchymal transition and immune evasion through its dynamic interactions with E2 enzymes and cullin-RING ligase complexes.
Picture
Picture
Western Blot
WB result of ARIH1 Recombinant Rabbit mAb
Primary antibody: ARIH1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 64 kDa
Observed MW: 64 kDa
WB result of ARIH1 Recombinant Rabbit mAb
Primary antibody: ARIH1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 64 kDa
Observed MW: 64 kDa
WB result of ARIH1 Recombinant Rabbit mAb
Primary antibody: ARIH1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 64 kDa
Observed MW: 64 kDa
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti- ARIH1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in NIH/3T3 cells. Anti- ARIH1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
