ARHGAP25 Recombinant Rabbit mAb (S-4005-5)

Catalog Number: S0B6966 Application: WB, IHC-P Reactivity: Hu Conjugation: Unconjugated Brand: Starter

Price:

$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	ARHGAP25
Synonyms	Rho GTPase-activating protein 25; KIAA0053
Immunogen	Synthetic Peptide
Accession	P42331
Clone Number	S-4005-5
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu
Positive Sample	Ramos, Raji, Jurkat, Daudi, THP-1
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:500-1:1000	Hu
IHC-P	1:500	Hu

Background

ARHGAP25, also known as Rho GTPase-activating protein 25, is a critical regulatory protein belonging to the ARHGAP family that functions primarily as a GTPase-activating protein (GAP) for the Rho family of small GTPases, specifically targeting Rac1 and Cdc42 to accelerate their conversion from the active GTP-bound state to the inactive GDP-bound state, thereby negatively regulating cytoskeletal dynamics, cell migration, and phagocytosis. Predominantly expressed in hematopoietic cells, particularly neutrophils and macrophages, ARHGAP25 plays a pivotal role in modulating immune responses by controlling reactive oxygen species (ROS) production via the NADPH oxidase complex and regulating the formation of lamellipodia and filopodia, which are essential for cell motility and pathogen engulfment; furthermore, its activity is tightly regulated through interactions with various signaling molecules and post-translational modifications, ensuring precise spatial and temporal control over Rho GTPase signaling pathways, which, when dysregulated, can contribute to inflammatory diseases, immunodeficiencies, or cancer metastasis, highlighting its significance as a potential therapeutic target in conditions involving aberrant cell movement and immune dysfunction.

Picture

Western Blot

WB result of ARHGAP25 Recombinant Rabbit mAb
Primary antibody incubation conditions: overnight at 4°C
Primary antibody: ARHGAP25 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PANC-1 whole cell lysate 20 µg
Lane 2: Ramos whole cell lysate 20 µg
Lane 3: Raji whole cell lysate 20 µg
Lane 4: Jurkat whole cell lysate 20 µg
Lane 5: Daudi whole cell lysate 20 µg
Lane 6: THP-1 whole cell lysate 20 µg
Negative control: PANC-1 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 73 kDa
Observed MW: 77 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human spleen. Anti-ARHGAP25 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human tonsil. Anti-ARHGAP25 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-ARHGAP25 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-ARHGAP25 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.