WB result of Arginase-1 Rabbit mAb
Primary antibody: Arginase-1 Rabbit mAb at 1/500 dilution
Lane 1: A549 whole cell lysate 40 µg
Lane 2: HepG2 whole cell lysate 40 µg
Negative control: A549 whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 35 kDa
Observed MW: 37 kDa
Exposure time: 180 s
Arginase-1 Recombinant Rabbit mAb (SDT-369-33)
Arginase-1 Recombinant Rabbit mAb (SDT-369-33)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Arginase-1 |
| Synonyms | Liver-type arginase; Type I arginase; ARG-1 |
| Location | Cytoplasm |
| Accession | P05089 |
| Clone Number | SDT-369-33 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IP, IHC-P, ICC |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.25 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:500 | |
| IP | 1:25 | |
| IHC | 1:1000 | |
| ICC | 1:50 |
Background
Arginase belongs to the urea hydrolase family of enzymes. It's one of the key enzymes in the urea nitrogen cycle and catalyzes the fifth and final step in the urea cycle, a series of biochemical reactions in mammals during which the body disposes of harmful ammonia. Specifically, arginase converts L-arginine into L-ornithine and urea. It was found to be highly specific in normal liver cells, but not in bile duct epithelial cells, hepatic sinus endothelial cells, vascular endothelial cells, and Kupffer cells. It is a specific and sensitive marker for hepatocellular adenoma and hepatocellular carcinoma (more sensitive and specific than HepPar1 and Glypican-3).
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Western Blot
IP
Arginase-1 Rabbit mAb at 1/25 dilution (1 µg) immunoprecipitating Arginase-1 in 0.4 mg HepG2 whole cell lysate.
Western blot was performed on the immunoprecipitate using Arginase-1 Rabbit mAb at 1/500 dilution.
Secondary antibody (HRP) for IP was used at 1/400 dilution.
Lane 1: HepG2 whole cell lysate 20 µg (Input)
Lane 2: Arginase-1 Rabbit mAb IP in HepG2 whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in HepG2 whole cell lysate
Predicted MW: 35 kDa
Observed MW: 37 kDa
Exposure time: 180 s
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human liver. Anti-Arginase-1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human colon. Anti-Arginase-1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human kidney. Anti-Arginase-1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-Arginase-1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-Arginase-1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining on immune cells in paraffin-embedded human gastric carcinoma. Anti-Arginase-1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining on immune cells in paraffin-embedded human cervical squamous cell carcinoma. Anti-Arginase-1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HepG2 cells. Anti- Arginase-1 antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
