ARF6 Recombinant Rabbit mAb (S-4283-62)

Catalog Number: S0B60346 Application: WB, IHC-P Reactivity: Hu, Ms, Rt, Mk Conjugation: Unconjugated Brand: Starter

Price:

$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	ARF6
Synonyms	ADP-ribosylation factor 6
Immunogen	Synthetic Peptide
Location	Cytoplasm, Endosome, Cell membrane
Accession	P62330
Clone Number	S-4283-62
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu, Ms, Rt, Mk
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000-1:5000	Hu, Ms, Rt, Mk
IHC-P	1:2000	Hu

Background

ARF6 (ADP-ribosylation factor 6) is a small GTPase belonging to the ARF family that functions as a critical molecular switch regulating plasma membrane dynamics, endosomal trafficking, and actin cytoskeleton remodeling by cycling between active GTP-bound and inactive GDP-bound states. Unlike other ARF isoforms primarily associated with Golgi transport, ARF6 uniquely localizes to the plasma membrane and endosomes, where it orchestrates clathrin-independent endocytosis, recycling of membrane proteins such as integrins and MHC class I molecules, and the formation of membrane ruffles and lamellipodia through downstream effectors like JIP3/4, POR1, and phospholipase D. Its activity is tightly controlled by guanine nucleotide exchange factors (GEFs) such as EFA6 and cytohesins, as well as GTPase-activating proteins (GAPs) including ACAPs and GITs, ensuring precise spatiotemporal regulation of cellular processes vital for cell migration, adhesion, phagocytosis, and pathogen entry, while its dysregulation has been implicated in cancer metastasis, neurological disorders, and infectious diseases due to aberrant membrane trafficking and cytoskeletal reorganization.

Picture

Western Blot

WB result of ARF6 Recombinant Rabbit mAb
Primary antibody: ARF6 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: 293T whole cell lysate 20 µg
Lane 2: MCF7 whole cell lysate 20 µg
Lane 3: A-431 whole cell lysate 20 µg
Lane 4: HeLa whole cell lysate 20 µg
Lane 5: Hep G2 whole cell lysate 20 µg
Lane 6: A549 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 20 kDa
Observed MW: 20 kDa

WB result of ARF6 Recombinant Rabbit mAb
Primary antibody: ARF6 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: RAW 264.7 whole cell lysate 20 µg
Lane 2: NIH/3T3 whole cell lysate 20 µg
Lane 3: mouse kidney lysate 20 µg
Lane 4: mouse liver lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 20 kDa
Observed MW: 20 kDa

WB result of ARF6 Recombinant Rabbit mAb
Primary antibody: ARF6 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: rat kidney lysate 20 µg
Lane 3: rat liver lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 20 kDa
Observed MW: 20 kDa

WB result of ARF6 Recombinant Rabbit mAb
Primary antibody: ARF6 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 20 kDa
Observed MW: 20 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human kidney. Anti-ARF6 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human stomach. Anti-ARF6 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human breast cancer. Anti-ARF6 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-ARF6 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti-ARF6 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.