2μg (R: reducing condition, N: non-reducing condition).
APRIL/TNFSF13 Protein, Human
APRIL/TNFSF13 Protein, Human
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | Tumor necrosis factor ligand superfamily member 13, A proliferation-inducing ligand (APRIL), TNF- and APOL-related leukocyte expressed ligand 2 (TALL-2), TNF-related death ligand 1 (TRDL-1), CD256 |
| Accession | O75888 |
| Amino Acid Sequence | Gln111-Leu250 |
| Expression System | HEK293 |
| Molecular Weight | 18-20 kDa (Reducing) |
| Purity | >95% by SDS-PAGE; >90% by HPLC. |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Physical Appearance | Lyophilized powder |
| Storage Buffer | 20mM Tris, 300mM NaCl, pH8.0 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | ·12 months from date of receipt, lyophilized powder stored at -20 to -80℃. |
| Reference | Nature. 2021 Sep;597(7874):92-96. |
Background
APRIL (A Proliferation-Inducing Ligand), also known as TNFSF13, is a member of the tumor necrosis factor (TNF) superfamily encoded by the TNFSF13 gene located on human chromosome 17p13. This 250-amino acid type II transmembrane protein functions as a homotrimeric cytokine and plays pivotal roles in immune regulation, B cell biology, and various pathological conditions including cancer, autoimmune diseases, and cardiovascular disorders.
APRIL is extensively studied in systemic lupus erythematosus (SLE), Sjögren's syndrome, IgA nephropathy, and rheumatoid arthritis. Elevated APRIL levels correlate with disease activity, making it a therapeutic target. Atacicept (TACI-Ig fusion protein) neutralizes both APRIL and BAFF, showing promise in clinical trials.
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SDS-PAGE
RP-HPLC
>90% as determined by RP-HPLC.
ELISA
Immobilized APRIL/TNFSF13 Protein, Human (Cat. No. UA040509) at 2.0μg/mL (100μL/well) can bind TACI/CD267/TNFRSF13B Fc Chimera Protein, Human (Cat. No. UA011118) with EC50 of 17.09-25.91 ng/mL.
