APOL2 Recombinant Rabbit mAb (S-3627-115)

Catalog Number: S0B6823 Application: WB, IHC-P Reactivity: Hu Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	APOL2
Synonyms	Apolipoprotein L2
Immunogen	Synthetic Peptide
Location	Cytoplasm
Accession	Q9BQE5
Clone Number	S-3627-115
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu
Positive Sample	SK-OV-3, Saos-2, RPMI-8226
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Hu
IHC-P	1:250	Hu

Background

Apolipoprotein L2 (APOL2) is a member of the apolipoprotein L family, primarily synthesized in the liver and secreted into the bloodstream as a component of high-density lipoprotein (HDL) particles, where it plays a significant role in lipid metabolism and reverse cholesterol transport. Structurally, APOL2 shares homology with other APOL proteins, containing an N-terminal signal peptide, a central membrane-associating domain, and a C-terminal SRA-interacting domain, although unlike its paralog APOL1, it does not exhibit trypanolytic activity against Trypanosoma brucei rhodesiense. Beyond its metabolic functions, emerging research suggests that APOL2 may be involved in cellular processes such as autophagy and inflammation, potentially interacting with intracellular membranes to influence vesicle trafficking or serving as a biomarker in certain pathological conditions including kidney disease and cancer, though its precise mechanistic contributions to these non-lipid pathways remain an active area of investigation compared to the more extensively characterized roles of other family members.

Picture

Western Blot

WB result of APOL2 Recombinant Rabbit mAb
Primary antibody: APOL2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: THP-1 whole cell lysate 20 µg
Lane 2: SK-OV-3 whole cell lysate 20 µg
Lane 3: Saos-2 whole cell lysate 20 µg
Low expression control: THP-1 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 37 kDa
Observed MW: 37 kDa

WB result of APOL2 Recombinant Rabbit mAb
Primary antibody: APOL2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: RPMI-8226 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 37 kDa
Observed MW: 37 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human liver. Anti-APOL2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human placenta. Anti-APOL2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human testis. Anti-APOL2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.