WB result of APOBEC3B Rabbit pAb
Primary antibody: APOBEC3B Rabbit pAb at 1/1000 dilution
Lane 1: SK-BR-3 whole cell lysate 20 µg
Lane 2: A431 whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Lane 4: HeLa whole cell lysate 20 µg
Lane 5: HCT 116 whole cell lysate 20 µg
Lane 6: HaCaT whole cell lysate 20 µg
Lane 7: HCC827 whole cell lysate 20 µg
Lane 8: U-2 OS whole cell lysate 20 µg
Negative control: SK-BR-3 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 46 kDa
Observed MW: 35 kDa
APOBEC3B Rabbit Polyclonal Antibody
APOBEC3B Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | APOBEC3B |
| Synonyms | DNA dC->dU-editing enzyme APOBEC-3B; A3B; Phorbolin-1-related protein; Phorbolin-2/3 |
| Immunogen | Recombinant Protein |
| Location | Nucleus |
| Accession | Q9UH17 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu |
| Positive Sample | A431, HepG2, HeLa, HCT 116, HaCaT, HCC827, U-2 OS |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| ICC | 1:500 | Hu |
Background
APOBEC3B (Apolipoprotein B mRNA editing enzyme catalytic subunit 3B) is a member of the human APOBEC (Apolipoprotein B mRNA editing enzyme catalytic polypeptide-like) family, which includes enzymes that deaminate cytosine (C) to uracil (U) in RNA and single-stranded DNA. APOBEC3B is a DNA cytosine deaminase that can convert cytosine in DNA to uracil. This conversion, if not repaired, can lead to C to T transition mutations during DNA replication. Its role in tumorigenesis in cancer development is achieved by promoting mutation processes. These mutations provide genetic diversity for cancer, thereby promoting cancer invasion, metastasis, recurrence, and therapeutic resistance.
Picture
Picture
Western Blot
Immunocytochemistry
ICC shows positive staining in A431 cells. Anti-APOBEC3B antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
