APC Recombinant Rabbit mAb

The Adenomatous polyposis coli (APC) protein is a large, multi-domain tumor suppressor encoded by the human APC gene; its canonical isoform contains 2,843 amino acids with a molecular weight of approximately 312 kDa. Its core function is to act as a negative regulator of the Wnt signaling pathway by serving as a scaffold for the degradation complex containing Axin and GSK3β, promoting the phosphorylation and proteasomal degradation of β-catenin and thereby suppressing excessive cell proliferation. Beyond this classical role, APC also directly or indirectly participates in the regulation of the cytoskeleton (e.g., microtubule stabilization and actin network organization), cell adhesion, migration, spindle formation, and chromosome segregation, among other processes. A substantial proportion of the protein is intrinsically disordered, a structural feature that enables it to interact flexibly with numerous binding partners (such as β-catenin, EB1, and IQGAP1) and may allow it to participate in signaling regulation through liquid–liquid phase separation. Germline mutations in the APC gene cause familial adenomatous polyposis (FAP), while somatic mutations occur in the majority of sporadic colorectal cancers, typically resulting in the production of truncated proteins and loss of function; this is an early key event in colorectal tumorigenesis.