Flow cytometric analysis of Mouse CD8β expression on C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes were stained with SDT Pacific Blue Rat Anti-Mouse CD3 Antibody (S0B8390) and either APC Rat IgG1, k Isotype Control (left panel) or SDT APC Rat Anti-Mouse CD8β Antibody (right panel) at 1.25 μl/test. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
APC Rat Anti-Mouse CD8β Antibody (53-5.8)
APC Rat Anti-Mouse CD8β Antibody (53-5.8)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | CD8β |
| Location | Membrane |
| Accession | P10300 |
| Clone Number | 53-5.8 |
| Antibody Type | Rat mAb |
| Isotype | IgG1,k |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | C57BL/6 mouse splenocytes |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | APC |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 0.25μg per million cells in 100μl volume | Ms |
Background
CD8β is a protein that, along with CD8α, forms the CD8 co - receptor complex on the surface of cytotoxic T cells. This complex plays a crucial role in the immune response by facilitating the interaction between cytotoxic T cells and antigen - presenting cells. CD8β helps stabilize the binding of the T cell receptor to MHC class I molecules on the antigen - presenting cell, thereby enhancing the specificity and efficiency of the T cell - mediated immune response. This interaction is essential for the activation of cytotoxic T cells, which can then target and destroy infected or malignant cells.
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