Flow cytometric analysis of Mouse CD38 expression on C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes were stained with FITC Rat Anti-Mouse CD3 Antibody (S0B1500) and either APC Rat IgG2a, κ Isotype Control (left panel) or SDT APC Rat Anti-Mouse CD38 Antibody (right panel) at 5 μl/test. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
APC Rat Anti-Mouse CD38 Antibody (NIMR5)
APC Rat Anti-Mouse CD38 Antibody (NIMR5)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | CD38 |
| Location | Membrane |
| Accession | P56528 |
| Clone Number | NIMR5 |
| Antibody Type | Rat mAb |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | C57BL/6 mouse splenocytes |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | APC |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1μg per million cells in 100μl volume | Ms |
Background
CD38 is a multifunctional type II transmembrane glycoprotein expressed on immune cells and in various tissues, acting both as a receptor and an enzyme that converts NAD⁺ into cyclic ADP-ribose and ADP-ribose to regulate intracellular calcium signaling, immune cell activation, proliferation, and migration, and is also a key therapeutic target in multiple myeloma due to its high expression on malignant plasma cells and its role in modulating NAD⁺ levels linked to aging and metabolic processes.
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