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APC Mouse Anti-Human CD68 Antibody (S-R657)

APC Mouse Anti-Human CD68 Antibody (S-R657)

Catalog Number: S0B8442 Application: FCM Reactivity: Human Conjugation: APC Brand: Starter
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Product Details

Product Specification


Host Mouse
Antigen CD68
Synonyms Macrosialin; Gp110
Location Cell membrane
Accession P34810
Clone Number S-R657
Antibody Type Mouse mAb
Isotype IgG2b,k
Application FCM
Reactivity Hu
Positive Sample Human PBMC
Purification Protein A
Concentration 0.2 mg/ml
Conjugation APC
Physical Appearance Liquid
Storage Buffer

PBS, 1% BSA, 0.3% Proclin 300
Stability & Storage

12 months from date of receipt / reconstitution, 2 to 8 °C as supplied

Dilution


application dilution species
ICFCM 1.25μl per million cells in 100μl volume Hu

Background

CD68 is a heavily glycosylated, 110 kDa type I transmembrane glycoprotein of the lysosome-associated membrane protein (LAMP) family (also called LAMP-4) that is highly expressed on human monocytes, macrophages, osteoclasts, and other myeloid cells, where it predominantly localizes to lysosomes and endosomes and participates in phagocytosis, debris clearance, and inflammatory responses via its scavenger-receptor activity, while alternative splicing produces multiple isoforms and its presence on tumor cells and ability to bind selectins implicate it in metastasis, making it a widely used histological macrophage marker.

Picture

FC

Human PBMC (human peripheral blood mononuclear cells) were fixed with 4% PFA and permeabilization with the 0.1% Tween. Cells were then intracellularly stained with Brilliant Violet 421™ Mouse Anti-Human CD11b and either APC Mouse IgG2b, κ Isotype Control / (Left panel) or APC Mouse Anti-Human CD68 Antibody at 1.25μl/test / (Right panel). Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.