Flow cytometric analysis of human CD123 expression on human PBMC (human peripheral blood mononuclear cell). Human PBMC were stained with Brilliant Violet 605™ Mouse Anti-Human HLA/DR antibody and either APC Mouse IgG2a, κ Isotype Control (Left panel) or SDT APC Mouse Anti-Human CD123 Antibody (Right panel) at 0.25 μg/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
APC Mouse Anti-Human/ Monkey CD123 Antibody (7G3)
APC Mouse Anti-Human/ Monkey CD123 Antibody (7G3)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD123 |
| Synonyms | Translation initiation factor eIF2 assembly protein; Cell division cycle protein 123 homolog (Protein D123); HT-1080; PZ32 |
| Location | Cytoplasm, Cell membrane |
| Accession | O75794 |
| Clone Number | 7G3 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2a,k |
| Application | FCM |
| Reactivity | Hu, RhMk, CyMk |
| Positive Sample | human PBMC |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | APC |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1.25μl per million cells in 100μl volume | Hu, RhMk, CyMk |
Background
The CD123 protein, also known as the Interleukin-3 receptor subunit alpha (IL-3RA) or CD123, is a crucial component of the interleukin-3 (IL-3) receptor complex and belongs to the Type I cytokine receptor family, specifically the Class 5 subfamily. CD123 serves as the high-affinity receptor for IL-3, forming a heterodimeric receptor complex with the β chain (CSF2RB). This protein mediates the stimulatory effects of IL-3 on the proliferation and differentiation of various hematopoietic cells, including pluripotent hematopoietic stem cells and lineage-committed cells. CD123 is expressed on normal plasmacytoid dendritic cells, basophils, monocytes, and eosinophils, and is overexpressed in various hematologic malignancies. In clinical research, the expression of CD123 is associated with chemotherapy resistance and high-risk genetic alterations in certain leukemias, such as acute myeloid leukemia (AML).
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Flow cytometric analysis of CD123 expression on Rhesus monkey peripheral blood cells. Rhesus macaque peripheral blood cells were stained with APC Mouse IgG2a, κ Isotype Control (left panel) or SDT APC Mouse Anti- Human CD123 Antibody (right panel) at 0.25 μg/test. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Flow cytometric analysis of CD123 expression on Cynomolgus monkey peripheral blood cells. Cynomolgus macaque peripheral blood cells were stained with APC Mouse IgG2a, κ Isotype Control (left panel) or SDT APC Mouse Anti- Human CD123 Antibody (right panel) at 0.25 μg/test. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
