Flow cytometric analysis of CD90.2 (Thy-1.2) expression on BALB/c mouse splenocytes. BALB/c mouse splenocytes were stained with Brilliant Violet 421™ Rat Anti-Mouse CD3 Antibody and either APC-Cy7 Rat IgG2a, κ Isotype Control (left panel) or SDT APC-Cy7 Rat anti-Mouse CD90.2 (Thy-1.2) Antibody (right panel) at 5 μl/test with True-Stain Monocyte Blocker™. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
APC-Cy7 Rat anti-Mouse CD90.2 (Thy-1.2) Antibody (53-2.1)
APC-Cy7 Rat anti-Mouse CD90.2 (Thy-1.2) Antibody (53-2.1)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | CD90.2 (Thy-1.2) |
| Synonyms | Thy-1 membrane glycoprotein; Thy-1 antigen; CD90; Thy-1; Thy1 |
| Location | Cell membrane |
| Accession | P01831 |
| Clone Number | 53-2.1 |
| Antibody Type | Rat mAb |
| Isotype | IgG2a,k |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | BALB/c mouse splenocytes |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | APC-Cy7 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1μg per million cells in 100μl volume | Ms |
Background
CD90.2 (Thy-1.2) is a 25-35 kDa glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein and a member of the immunoglobulin superfamily. It is primarily expressed on hematopoietic stem cells, neurons, all thymocytes, and peripheral T cells in mouse strains such as BALB/c, C57BL/6, and C3H. This molecule plays a role in cell adhesion, signal transduction, and the regulation of T cell activation. Additionally, CD90.2 is involved in lymphocyte proliferation and hematopoietic stem cell differentiation. It is also notable for its interaction with CD45 and its potential role in cell-cell interactions during synaptogenesis in the brain.
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