WB result of AP-2α Recombinant Rabbit mAb
Primary antibody: AP-2α Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 μg
Lane 2: MCF7 whole cell lysate 20 μg
Lane 3: SH-SY5Y whole cell lysate 20 μg
Lane 4: 293T whole cell lysate 20 μg
Lane 5: Hep G2 whole cell lysate 20 μg
Lane 6: A431 whole cell lysate 20 μg
Lane 7: SW480 whole cell lysate 20 μg
Lane 8: SK-OV-3 whole cell lysate 20 μg
Lane 9: IMR-32 whole cell lysate 20 μg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 108 kDa
Observed MW: 108 kDa
AP-2α Recombinant Rabbit mAb (S-4444-44)
AP-2α Recombinant Rabbit mAb (S-4444-44)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | AP-2α |
| Synonyms | Transcription factor AP-2-alpha; AP2-alpha; AP-2 transcription factor; Activating enhancer-binding protein 2-alpha; Activator protein 2 (AP-2); AP2TF; TFAP2; TFAP2A |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | P05549 |
| Clone Number | S-4444-44 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, MCF7, SH-SY5Y, 293T, HepG2, A431, SW480, SK-OV-3, IMR-32, NIH/3T3, F9, mouse brain, rat brain |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:2000 | Hu, Ms, Rt |
| IHC-P | 1:500-1:2000 | Hu, Ms, Rt |
Background
AP-2α protein, full name Activating Enhancer Binding Protein 2 Alpha, is encoded by the TFAP2A gene located on human chromosome 6pter-p22.3. It is the earliest discovered and most extensively studied member of the AP-2 transcription factor family, belonging to a class of sequence-specific DNA-binding proteins whose expression is induced by signals such as retinoic acid during development. Structurally, the AP-2α protein contains a proline- and glutamine-rich transcriptional activation domain at its N-terminus, while the highly conserved C-terminus comprises a basic DNA-binding motif and a unique "helix-span-helix" dimerization domain, enabling it to form homodimers or heterodimers that specifically recognize and bind the conserved sequence 5'-GCCNNNGGC-3' in the promoter regions of target genes, thereby regulating downstream gene transcription. Functionally, AP-2α plays an irreplaceable role in embryonic development, with its expression first appearing in tissues such as the neural crest and limb bud mesenchyme; knockout mice exhibit embryonic lethality due to severe craniofacial, limb, and neural tube defects, underscoring its critical regulatory functions in cell proliferation, differentiation, and apoptosis. Furthermore, numerous studies have shown that AP-2α expression is frequently downregulated in various human cancers, including breast cancer, ovarian cancer, colorectal cancer, and melanoma, and it exerts its functions by activating growth-suppressive genes such as p21, leading to its widespread recognition as an important tumor suppressor gene.
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Picture
Western Blot
WB result of AP-2α Recombinant Rabbit mAb
Primary antibody: AP-2α Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 μg
Lane 2: F9 whole cell lysate 20 μg
Lane 3: mouse brain lysate 20 μg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 108 kDa
Observed MW: 108 kDa
WB result of AP-2α Recombinant Rabbit mAb
Primary antibody: AP-2α Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat brain lysate 20 μg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 108 kDa
Observed MW: 108 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti- AP-2α antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti- AP-2α antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti- AP-2α antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti- AP-2α antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti- AP-2α antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti- AP-2α antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti- AP-2α antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
