ELISA binding of Anti-Pembrolizumab Recombinant Rabbit mAb (SDT-825-21-1) with Pembrolizumab. While the antibody does not recognize the human IgG4 control and human total IgG (data not shown).
Coating antigen: Pembrolizumab, 0.2 µg/ml.
Anti-Pembrolizumab Recombinant Rabbit mAb (SDT-825-21-1) (S0B0843) dilutions start from 500 ng/ml.
EC50= 3.983 ng/ml.
Anti-Pembrolizumab Recombinant Rabbit mAb (SDT-825-21-1)
Anti-Pembrolizumab Recombinant Rabbit mAb (SDT-825-21-1)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Pembrolizumab |
| Synonyms | Keytruda |
| Clone Number | SDT-825-21-1 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | ELISA, Competitive ELISA, PK study |
| Purification | Protein A |
| Conjugation | Unconjugated |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | Lyophilized with PBS pH7.4, 0.03% Proclin 300 |
| Reconstitution | Reconstitute the lyophilized powder with deionized water to a final concentration of 0.5 mg/ml. |
| Stability & Storage | The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles. |
Dilution
| application | dilution | species |
| ELISA | 0.005-0.05 µg/ml |
Background
Pembrolizumab, sold under the brand name Keytruda, is a humanized antibody used in cancer immunotherapy that treats melanoma, lung cancer, head and neck cancer, Hodgkin lymphoma, stomach cancer, cervical cancer, and certain types of breast cancer. It is administered by slow intravenous injection. It is an IgG4 isotype antibody that blocks a protective mechanism of cancer cells, allowing the immune system to destroy them. It targets the programmed cell death protein 1 (PD-1) receptor of lymphocytes by blocking its interaction with PD-L1, resulting in the activation of T-cell-mediated immune responses against tumor cells.
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ELISA
Compete ELISA
Anti-Pembrolizumab Recombinant Rabbit mAb (SDT-825-21-1) (S0B0843) blocks Pembrolizumab binding with PD-1 Fc Chimera Protein, Human (UA010002, STARTER).
Coating antigen: Pembrolizumab, 1 µg/ml
PD-1-Fc-biotin final concentration: 5 ng/ml
Anti-Pembrolizumab Recombinant Rabbit mAb (SDT-825-21-1) (S0B0843) dilutions start from 40 μg/ml.
IC50= 344 ng/ml.
Paired Recommendations
Standard curve of Pembrolizumab Sandwich ELISA. The Pembrolizumab Sandwich ELISA assay is developed by using Anti-Pembrolizumab Recombinant Rabbit mAb (SDT-825-61) (S0B0842) and Anti-Pembrolizumab Recombinant Rabbit mAb (SDT-825-21-1) (S0B0843) as the capture and detection antibodies, respectively.
In this ELISA assay, Anti-Pembrolizumab Recombinant Rabbit mAb (SDT-825-21-1) (S0B0843) was labeled with Biotin.
The sensitivity of detecting Pembrolizumab is up to 29 pg/ml.
