Flow cytometric analysis of 2C6 expression on CAR (2C6 scFv) transfected 293F (human embryonic kidney epithelial cell) (right panel) or 293F (left panel) was stained with anti-human 2C6 scFv (Anti-CD20 CAR-T) Recombinant Rabbit mAb (Alexa Fluor® 488 Conjugate) at 2 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Anti-Human 2C6 scFv (Anti-CD20 CAR-T) Recombinant Rabbit mAb (Alexa Fluor® 488 Conjugate) (S-5413)
Anti-Human 2C6 scFv (Anti-CD20 CAR-T) Recombinant Rabbit mAb (Alexa Fluor® 488 Conjugate) (S-5413)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Clone Number | S-5413 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Isotype Control | S0B0161 |
| Application | FCM |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.1 mg/ml |
| Conjugation | Alexa Fluor® 488 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 2μl per million cells in 100μl volume | Hu |
Background
This antibody is a critical detection tool. Its core function is to specifically recognize and bind the 2C6 single-chain variable fragment (scFv) displayed on the surface of Anti-CD20 CAR-T cells. This 2C6 scFv is the structural domain on the CAR-T cell responsible for targeting and binding the CD20 antigen, and this antibody serves as its anti-idiotypic antibody, precisely binding to this engineered CAR molecule with high specificity, much like a "key fitting a lock." Therefore, it is commonly used as a companion diagnostic reagent in CAR-T therapy clinical trials to detect the survival, proliferation, and expression levels of infused CAR-T cells in patients via methods such as flow cytometry.
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