Anti-GFP Nanobody Agarose Beads is used to immunoprecipitated GFP-fusion proteins from Escherichia coli lysate supernatant. During elution with Acidic elution buffer, the enriched GFP-fusion proteins are released from Anti-GFP Nanobody Agarose Beads.
SDS-PAGE analysis was performed.
Lane 1: Input
Lane 2: Flow-through
Lane 3: GFP-fusion protein eluted by acidic buffer
Lane 4: Agarose Beads after elution
Anti-GFP Nanobody Agarose Beads
Anti-GFP Nanobody Agarose Beads
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$585.00 USD
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$585.00 USD
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Product Details
Product Details
Product Specification
| Antigen | GFP |
| Synonyms | Green fluorescent protein |
| Application | IP, Protein purification, Co-IP |
| Cross Reactivity |
Can also enrich GFP variants such as CFP, ECFP, YFP, BFP, aceGFP, EGFP, Clover, Citrine, EYFP and Venus.
|
| Physical Appearance | Liquid |
| Storage Buffer | 50% slurry in PBS pH7.4, 0.05% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| IP | 25ul/test |
Background
Anti-GFP nanobody agarose beads is an affinity chromatography medium designed for easy, one-step purification of recombinant GFP fusion proteins in E. coli, insect cells and mammalian cells. The recombinant GFP fusion proteins can be purified directly from pre-treated cell lysate using Anti-GFP nanobody agarose beads. It is the excellent choice for high performance purifications.
Picture
Picture
SDS-PAGE
