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Anti-FMC63 Recombinant Mouse mAb (S-5031)

Anti-FMC63 Recombinant Mouse mAb (S-5031)

Catalog Number: S0B6997 Application: FCM Brand: Starter
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Product Details

Product Specification


Host Mouse
Antigen FMC63 scFv
Clone Number S-5031
Antibody Type Mouse mAb
Isotype IgG1,k
Application FCM
Purification Protein G
Concentration 2 mg/ml
Conjugation Unconjugated
Physical Appearance Liquid
Storage Buffer

PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage

12 months from date of receipt / reconstitution, -20°C as supplied

Dilution


application dilution species
FCM 1:2000 Species independent

Background

FMC63 scFv (single-chain variable fragment) is a recombinant protein derived from the variable regions (VH and VL) of the anti-CD19 monoclonal antibody FMC63, linked by a flexible peptide spacer (e.g., (Gly₄Ser)₃). It specifically binds to the B-cell surface antigen CD19 with high affinity (KD ~1-10 nM) and is a key targeting component in FDA-approved CAR-T therapies, such as Kymriah®. The humanized FMC63 scFv retains potent antigen recognition while minimizing immunogenicity, making it highly effective in treating B-cell malignancies, including acute lymphoblastic leukemia (ALL) and non-Hodgkin lymphoma (NHL). Ongoing research focuses on optimizing its affinity, stability, and functionality to enhance the safety and efficacy of CAR-T cell therapies.

Picture

FC

Flow cytometric analysis of FMC63 expression on CAR (FMC63 scFv) transfected Jurkat (human T cell leukemia T lymphocyte). CAR (FMC63 scFv) transfected Jurkat (right panel) or Jurkat (left panel) were labeled with FMC63 antibody at 1/2000 dilution (0.1 μg). Goat Anti- Mouse IgG Alexa Fluor® 488 was used as the secondary antibody. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.